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Enrichment of Echinacea angustifolia with Bauer Alkylamide 11 and Bauer Ketone 23 Increased Anti-inflammatory Potential through Interference with COX-2 Enzyme Activity
Journal of Agricultural and Food Chemistry
  • Carlie A. LaLone, Iowa State University
  • Nan Huang, Iowa State University
  • Ludmila Rizshsky, Iowa State University
  • Man-Yu Yum, Iowa State University
  • Navrozedeep Singh, Iowa State University
  • Cathy Hauck, Iowa State University
  • Basil J. Nikolau, Iowa State University
  • Eve S. Wurtele, Iowa State University
  • Marian L. Kohut, Iowa State University
  • Patricia A. Murphy, Iowa State University
  • Diane F. Birt, Iowa State University
Document Type
Article
Publication Date
1-1-2010
DOI
10.1021/jf1014268
Abstract
Bauer alkylamide 11 and Bauer ketone 23 were previously found to be partially responsible forEchinacea angustifolia anti-inflammatory properties. This study further tested their importance using the inhibition of prostaglandin E2 (PGE2) and nitric oxide (NO) production by RAW264.7 mouse macrophages in the absence and presence of lipopolysaccharide (LPS) and E. angustifolia extracts, phytochemical enriched fractions, or pure synthesized standards. Molecular targets were probed using microarray, qRT-PCR, Western blot, and enzyme assays. Fractions with these phytochemicals were more potent inhibitors of LPS-induced PGE2 production than E. angustifolia extracts. Microarray did not detect changes in transcripts with phytochemical treatments; however, qRT-PCR showed a decrease in TNF-α and an increase of iNOS transcripts. LPS-induced COX-2 protein was increased by an E. angustifolia fraction containing Bauer ketone 23 and by pure phytochemical. COX-2 activity was decreased with all treatments. The phytochemical inhibition of PGE2 production byEchinacea may be due to the direct targeting of COX-2 enzyme.
Comments

Posted with permission from Journal of Agricultural and Food Chemistry 58, no. 15 (2010): 8573–8584, doi:10.1021/jf1014268. Copyright 2010 American Chemical Society.

Copyright Owner
American Chemical Society
Language
en
File Format
application/pdf
Citation Information
Carlie A. LaLone, Nan Huang, Ludmila Rizshsky, Man-Yu Yum, et al.. "Enrichment of Echinacea angustifolia with Bauer Alkylamide 11 and Bauer Ketone 23 Increased Anti-inflammatory Potential through Interference with COX-2 Enzyme Activity" Journal of Agricultural and Food Chemistry Vol. 58 Iss. 15 (2010) p. 8573 - 8584
Available at: http://works.bepress.com/diane_birt/8/