Characterizing the Metabolic Fingerprint and Anti-inflammatory Activity of Hypericum gentianoidesJournal of Agricultural and Food Chemistry
AbstractIn this paper we characterize the metabolic fingerprint and first reported anti-inflammatory activity of Hypericum gentianoides. H. gentianoides has a history of medical use by Native Americans, but it has been studied very little for biological activity. High-performance liquid chromatography (HPLC) and liquid chromatography−electrospray ionization−mass spectrometry (LC-ESI-MS) analyses of a methanol extract show that H. gentianoides contains a family of over nine related compounds that have retention times, mass spectra, and a distinctive UV absorption spectra characteristic of certain acyl-phloroglucinols. These metabolites are abundant relative to other secondary products present in H. gentianoides, accounting for approximately 0.2 g per gram of dry plant tissue. H. gentianoides methanol extracts and a specific semipreparative HPLC fraction from these extracts containing the putative acyl-phloroglucinols reduce prostaglandin E2 synthesis in mammalian macrophages.
Copyright OwnerAmerican Chemical Society
Citation InformationMatthew L. Hillwig, Kimberly D.P. Hammer, Diane F. Birt and Eve S. Wurtele. "Characterizing the Metabolic Fingerprint and Anti-inflammatory Activity of Hypericum gentianoides" Journal of Agricultural and Food Chemistry Vol. 56 Iss. 12 (2008) p. 4359 - 4366
Available at: http://works.bepress.com/diane_birt/7/