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Article
Analysis of Resistant Starches in Rat Cecal Contents Using Fourier Transform Infrared Photoacoustic Spectroscopy
Journal of Agricultural and Food Chemistry
  • Timothy J. Anderson, Iowa State University
  • Yongfeng Ai, Iowa State University
  • Roger W. Jones, Iowa State University
  • Robert S. Houk, Iowa State University
  • Jay-lin Jane, Iowa State University
  • Yinsheng Zhao, Iowa State University
  • Diane F. Birt, Iowa State University
  • John F. McClelland, Iowa State University
Document Type
Article
Publication Date
1-29-2013
DOI
10.1021/jf3042616
Abstract

Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) qualitatively and quantitatively measured resistant starch (RS) in rat cecal contents. Fisher 344 rats were fed diets of 55% (w/w, dry basis) starch for 8 weeks. Cecal contents were collected from sacrificed rats. A corn starch control was compared against three RS diets. The RS diets were high-amylose corn starch (HA7), HA7 chemically modified with octenyl succinic anhydride, and stearic-acid-complexed HA7 starch. To calibrate the FTIR-PAS analysis, samples from each diet were analyzed using an enzymatic assay. A partial least-squares cross-validation plot generated from the enzymatic assay and FTIR-PAS spectral results for starch fit the ideal curve with a R(2) of 0.997. A principal component analysis plot of components 1 and 2 showed that spectra from diets clustered significantly from each other. This study clearly showed that FTIR-PAS can accurately quantify starch content and identify the form of starch in complex matrices.

Comments

This article is from Journal of Agricultural and Food Chemistry, 61(2013):1818-1822. doi: 10.1021/jf3042616. Posted with permission

Rights
Reprinted with permission from Journal of Agricultural and Food Chemistry, 61(8):1818-1822. doi: 10.1021/jf3042616 . Copyright (2013) American Chemical Society.
Copyright Owner
American Chemical Society
Language
en
Date Available
2014/10/28
File Format
application/pdf
Citation Information
Timothy J. Anderson, Yongfeng Ai, Roger W. Jones, Robert S. Houk, et al.. "Analysis of Resistant Starches in Rat Cecal Contents Using Fourier Transform Infrared Photoacoustic Spectroscopy" Journal of Agricultural and Food Chemistry Vol. 61 Iss. 8 (2013) p. 1818 - 1822
Available at: http://works.bepress.com/diane_birt/15/