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Rosmarinic Acid in Prunella vulgaris Ethanol Extract Inhibits Lipopolysaccharide-Induced Prostaglandin E2 and Nitric Oxide in RAW 264.7 Mouse Macrophages
Journal of Agricultural and Food Chemistry
  • Nan Huang, Iowa State University
  • Cathy Hauck, Iowa State University
  • Man-Yu Yum, Iowa State University
  • Ludmila Rizshsky, Iowa State University
  • Mark P. Widrlechner, United States Department of Agriculture
  • Joe-Ann McCoy, United States Department of Agriculture
  • Patricia A. Murphy, Iowa State University
  • Philip M. Dixon, Iowa State University
  • Basil J. Nikolau, Iowa State University
  • Diane F. Birt, Iowa State University
Document Type
Article
Publication Date
1-1-2009
DOI
10.1021/jf9023728
Abstract
Prunella vulgaris has been used therapeutically for inflammation-related conditions for centuries, but systematic studies of its anti-inflammatory activity are lacking and no specific active components have been identified. In this study, water and ethanol extracts of four P. vulgaris accessions were applied to RAW 264.7 mouse macrophages, and the ethanol extracts significantly inhibited lipopolysaccharide (LPS)-stimulated prostaglandin E2 (PGE2) and nitric oxide (NO) production at 30 μg/mL without affecting cell viability. Extracts from different accessions of P. vulgaris were screened for anti-inflammatory activity to identify accessions with the greatest activity. The inhibition of PGE2 and NO production by selected extracts was dose-dependent, with significant effects seen at concentrations as low as 10 μg/mL. Fractionation of ethanol extracts from the active accession, Ames 27664, suggested fractions 3 and 5 as possible major contributors to the overall activity. Rosmarinic acid (RA) content inP. vulgaris was found to independently inhibit inflammatory response, but it only partially explained the extracts’ activity. LPS-induced cyclooxygenase-2 (COX-2) and nitric oxide synthase (iNOS) protein expression were both attenuated by P. vulgaris ethanol extracts, whereas RA inhibited only COX-2 expression.
Comments

Posted with permission from Journal of Agricultural and Food Chemistry 57, no. 22 (2009): 10579–10589, doi:10.1021/jf9023728. Copyright 2009 American Chemical Society.

Copyright Owner
American Chemical Society
Language
en
File Format
application/pdf
Citation Information
Nan Huang, Cathy Hauck, Man-Yu Yum, Ludmila Rizshsky, et al.. "Rosmarinic Acid in Prunella vulgaris Ethanol Extract Inhibits Lipopolysaccharide-Induced Prostaglandin E2 and Nitric Oxide in RAW 264.7 Mouse Macrophages" Journal of Agricultural and Food Chemistry Vol. 57 Iss. 22 (2009) p. 10579 - 10589
Available at: http://works.bepress.com/diane_birt/10/