Article
Transport of proteins into chloroplasts. Delineation of envelope "transit" and thylakoid "transfer" signals within the pre-sequences of three imported thylakoid lumen proteins
The Journal of Biological Chemistry
(1991)
Abstract
The targeting of cytosolically synthesized proteins into the thylakoid lumen is mediated by an aminoterminal pre-sequence consisting of an "envelope transit" and a "thylakoid transfer" signal in tandem. We have investigated the structural characteristics of several thylakoid transfer signals by determining the intermediate sites at which the stromal processing peptidase cleaves to remove the transit sequences. Using this approach we have found that the thylakoid transfer signals of Silene pratensis plastocyanin, 23-kDa oxygen-evolving complex protein from wheat, and 33-kDa oxygen-evolving complex protein from wheat, are 25, 39, and 48 residues in length, respectively. All of the transfer signals contain hydrophobic core sequences and a "-3,-1" motif reminiscent of those found in signal sequences, but the amino-terminal regions of the transfer signals of the 23- and 33-kDa proteins are both longer and more highly charged. The net charge of each amino-terminal region of the transfer sequences is +1, including the amino-terminal amino group. In each case, the stromal processing peptidase cleaves immediately after a positively charged residue, but otherwise the cleavage sites exhibit no common elements of either primary or secondary structure.
Disciplines
Publication Date
1991
Publisher Statement
This research was originally published in The Journal of Biological Chemistry. Bassham DC, Bartling D, Mould RM, Dunbar B, Weisbeek P, Herrmann RG, Robinson C. Transport of Proteins into Chloroplasts. Delineation of envelope" transit" and thylakoid "transfer" signals within the pre-sequences of three imported thylakoid lumen proteins. The Journal of Biological Chemistry. 1991; 266:23606-23610. © the American Society for Biochemistry and Molecular Biology
Citation Information
Diane C. Bassham, Dieter Bartling, Ruth M. Mould, Bryan Dunbar, et al.. "Transport of proteins into chloroplasts. Delineation of envelope "transit" and thylakoid "transfer" signals within the pre-sequences of three imported thylakoid lumen proteins" The Journal of Biological Chemistry Vol. 266 Iss. 35 (1991) p. 23606 - 23610 Available at: http://works.bepress.com/diane-bassham/9/