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Presentation
An Arabidopsis syntaxin homologue isolated by functional complementation of a yeast pep12 mutant
Proceedings of the National Academy of Sciences of the United States of America (1995)
  • Diane C. Bassham, Michigan State University
  • Susannah Gal, Michigan State University
  • Alexandre da Silva Conceição, Michigan State University
  • Natasha V. Raikhel, Michigan State University
Abstract
The syntaxin family of integral membrane proteins are thought to function as receptors for transport vesicles, with different isoforms of this family localized to various membranes throughout the cell. The yeast Pep12 protein is a syntaxin homologue which may function in the trafficking of vesicles from the trans-Golgi network to the vacuole. We have isolated an Arabidopsis thaliana cDNA by functional complementation of a yeast pep12 mutant. The Arabidopsis cDNA (aPEP12) potentially encodes a 31-kDa protein which is homologous to yeast Pep12 and to other members of the syntaxin family, indicating that this protein may function in the docking or fusion of transport vesicles with the vacuolar membrane in plant cells. Northern blot analysis indicates that the mRNA is expressed in all tissues examined, although at a very low level in leaves. The mRNA is found in all cell types in roots and leaves, as shown by in situ hybridization experiments. The existence of plant homologues of proteins of the syntaxin family indicates that the basic vesicle docking and fusion machinery may be conserved in plants as it is in yeast and mammals.
Publication Date
August, 1995
Comments
Copyright 1995 National Academy of Sciences
Citation Information
Diane C. Bassham, Susannah Gal, Alexandre da Silva Conceição and Natasha V. Raikhel. "An Arabidopsis syntaxin homologue isolated by functional complementation of a yeast pep12 mutant" Proceedings of the National Academy of Sciences of the United States of America (1995)
Available at: http://works.bepress.com/diane-bassham/11/