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Fulvic Acid Oxidation State Detection Using Fluorescence Spectroscopy
Environmental Sciences & Technology (2002)
  • Derek Lovley, University of Massachusetts - Amherst
  • Lisa Klapper
  • Diane M McKnight
  • J Robin Fulton
  • Elizabeth L Blunt-Harris
  • Kelly P Nevin
  • Patrick G Hatcher
Abstract

Humic substances are a heterogeneous class of moderate molecular weight, yellow-colored biomolecules present in all soils, sediments, and natural waters. Although humic substances are generally resistant to microbial degradation under anaerobic conditions, some microorganisms in soils and sediments can use quinone moieties in humic substances as electron acceptors. Laboratory experiments have shown that humic substances can act as electron shuttles in the microbial reduction of ferric iron. Field studies of electron shuttling processes have been constrained by the lack of methods to characterize the oxidation state of quinone moieties in humic substances at natural concentrations. All humic substances have fluorescent properties, and fluorescence spectroscopy can indicate differences in precursor organic source of humic substances. Here we show that the quinone moieties responsible for electron transfer reactions contribute significantly to the fluorescence of humic substances. Further we use fluorescence spectroscopy to elucidate the oxidation state of quinone moieties in humic substances at natural concentrations found in sediment interstitial waters.

Disciplines
Publication Date
2002
Publisher Statement
DOI: 10.1021/es0109702
Citation Information
Derek Lovley, Lisa Klapper, Diane M McKnight, J Robin Fulton, et al.. "Fulvic Acid Oxidation State Detection Using Fluorescence Spectroscopy" Environmental Sciences & Technology Vol. 36 (2002)
Available at: http://works.bepress.com/derek_lovley/204/