Skip to main content
Growth of Geobacter Sulfurreducens Under Nutrient-Limiting Conditions in Continuous Culture
Environmental Microbiology (2005)
  • Derek Lovley, University of Massachusetts - Amherst
  • Abraham Esteve-Núñez
  • Mary Rothermich
  • Manju Sharma

A system for growing Geobacter sulfurreducens under anaerobic conditions in chemostats was developed in order to study the physiology of this organism under conditions that might more closely approximate those found in the subsurface than batch cultures. Geobacter sulfurreducens could be cultured under acetate-limiting conditions with fumarate or Fe(III)-citrate as the electron acceptor at growth rates between 0.04 and 0.09 h(-1). The molar growth yield was threefold higher with fumarate as the electron acceptor than with Fe(III), despite the lower mid-point potential of the fumarate/succinate redox couple. When growth was limited by availability of fumarate, high steady-state concentrations were detected, suggesting that fumarate is unlikely to be an important electron acceptor in sedimentary environments. The half-saturation constant, Ks, for acetate in Fe(III)-grown cultures (10 microM) suggested that the growth of Geobacter species is likely to be acetate limited in most subsurface sediments, but that when millimolar quantities of acetate are added to the subsurface in order to promote the growth of Geobacter for bioremediation applications, this should be enough to overcome any acetate limitations. When the availability of electron acceptors, rather than acetate, limited growth, G. sulfurreducens was less efficient in incorporating acetate into biomass but had higher respiration rates, a desirable physiological characteristic when adding acetate to stimulate the activity of Geobacter species during in situ uranium bioremediation. These results demonstrate that the ability to study the growth of G. sulfurreducens under steady-state conditions can provide insights into its physiological characteristics that have relevance for its activity in a diversity of sedimentary environments.

Publication Date
Publisher Statement
DOI: 10.1111/j.1462-2920.2005.00731.x
Citation Information
Derek Lovley, Abraham Esteve-Núñez, Mary Rothermich and Manju Sharma. "Growth of Geobacter Sulfurreducens Under Nutrient-Limiting Conditions in Continuous Culture" Environmental Microbiology Vol. 7 Iss. 5 (2005)
Available at: