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An Impact of the Trivedi Effect® - Biofield Energy Healing Based Herbomineral Formulation on Pro-inflammatory Cytokines Expression in Mouse Splenocytes
American Journal of Life Sciences (2016)
  • Mahendra Kumar Trivedi, Trivedi Global Inc.
  • Alice Branton, Trivedi Global Inc.
  • Dahryn Trivedi, Trivedi Global Inc.
  • Gopal Nayak, Trivedi Global Inc.
  • Ariadne Esmene Afaganis, Trivedi Global Inc.
  • Barbara Marie Bader, Trivedi Global Inc.
  • Brian A. Weekes, Trivedi Global Inc.
  • Daphne Luisa Dumas, Trivedi Global Inc.
  • Denise Marie Fiedler, Trivedi Global Inc.
  • Dennille Mellesia Smith, Trivedi Global Inc.
  • Desi Pano, Trivedi Global Inc.
  • Donna Felice Galla, Trivedi Global Inc.
  • Donna Maria Alija, Trivedi Global Inc.
  • Elaine Barbara Mullins, Trivedi Global Inc.
  • Elaine M. Scorza, Trivedi Global Inc.
  • Ellia O'Donnell, Trivedi Global Inc.
  • Fabio Massimo Paciucci, Trivedi Global Inc.
  • Frances Goodman Warlick, Trivedi Global Inc.
  • Haddon Norman Salt, Trivedi Global Inc.
  • Inthirani Arul, Trivedi Global Inc.
  • Jacqueline Y. Andrews, Trivedi Global Inc.
  • James Jay McLeran, Trivedi Global Inc.
  • James Stephen Burnett, Trivedi Global Inc.
  • Jean Caroline White, Trivedi Global Inc.
  • Mayank Gangwar, Trivedi Science Research Laboratory Pvt. Ltd.
  • Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd.
Due to the increased popularity of herbomineral preparations in the healthcare sector, a new proprietary herbomineral formulation was formulated consisting of ashwagandha root extract and three minerals viz. zinc chloride, magnesium gluconate, and sodium selenate. The objective of the study was to evaluate the in vitro effect of Biofield Energy Healing (The Trivedi Effect®) on the test formulation using murine splenocyte cells. The herbomineral formulation was divided into two parts; one defined as the control, while the other part was treated with the Biofield Energy Healing Treatment performed from a remote distance by twenty renowned Biofield Energy Healers (The Trivedi Effect®) and defined as the Biofield Treated formulation. The splenocyte cells were exposed to test formulations at concentration from 0.00001053 to 10.53 µg/mL and were analyzed after 48 hours for cell viability using MTT assay. The expression of the cytokines (TNF-α, IFN-γ, IL-1β, and MIP-1α) was determined using ELISA assay. The cell viability data showed that all the tested concentration ranges were found to be safe with percentage cell viability at more than 80%. Further, TNF-α expression was significantly inhibited in the Biofield Treated test formulation group with respect to the vehicle control, while at 0.001053 and 0.1053 µg/mL, the expression was suppressed by 1.70% and 8.16%, respectively in the Biofield Treated test formulation compared to the untreated formulation. However, a significant immunosuppression was reported in IFN-γ expression at 0.00001053, 0.0001053, 0.01053, 0.1053, and 1.053 µg/mL by 12.63%, 2.31%, 8.31%, 9.15%, and 7.86%, respectively in the Biofield Treated test formulation compared with the untreated test formulation. The MIP-1α expression was inhibited by 8.31%, 21.53%, and 8.70% at 0.0001053, 0.01053, and 0.1053 µg/mL, respectively in the Biofield Treated formulation compared with the untreated test formulation. However, IL-1β expression was significantly suppressed by 19.72% at concentration 0.00001053 µg/mL in the Biofield Treated test formulation compared with the untreated test formulation. Thus, the down-regulation of tested cytokines and chemokines in the Biofield Energy Healing test formulation might be applicable for controlling acute and chronic inflammation in many clinical diseases. Overall, the results demonstrated that The Trivedi Effect®- Biofield Energy Healing (TEBEH) has the capacity to potentiate the immunomodulatory activity of the test formulation, which can be useful against autoimmune disorders. Biofield Treated Test formulation may also be useful in anti-aging, anti-inflammatory, stress management and in preventing immune-mediated tissue damage in organ transplants by improving overall health and quality of life.

  • Biofield Energy Healing Treatment,
  • The Trivedi Effect®,
  • Herbomineral Formulation,
  • Immune-Modulation,
  • Pro-inflammatory Cytokines,
  • Splenocytes
Publication Date
December 8, 2016
Citation Information
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Mayank Gangwar, Snehasis Jana, An Impact of the Trivedi Effect® - Biofield Energy Healing Based Herbomineral Formulation on Pro-inflammatory Cytokines Expression in Mouse Splenocytes, American Journal of Life Sciences. Vol. 4, No. 6, 2016, pp. 164-174. doi: 10.11648/j.ajls.20160406.14
Creative Commons license
Creative Commons License
This work is licensed under a Creative Commons CC_BY International License.