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Article
Molecular cloning of the rat TA1/LAT-1/CD98 light chain gene promoter1
Faculty Publications
  • Deborah E Britt, Rhode Island College
  • Sri K. Diah, Division of Medical Oncology, Rhode Island Hospital/Brown Medical School
  • James F. Padbury, Department of Pediatrics, Rhode Island Hospital/Brown Medical School
Document Type
Article
Abstract
The rat LAT-1 (L-amino acid transporter-1) gene is a CD98 light chain highly expressed in cancer and development. As an initial study of the molecular basis underlying regulation of its expression, we cloned 2 kb of the LAT-1 5' flanking region. Inverse RACE and primer extension methods were used to define the transcription initiation site at 80 bp upstream from the translational start site. Functional studies carried out in normal hepatic cells using constructs containing progressive 5'deletion from region -1958 to -185 showed 3-5-fold beta-galactosidase activities over control. The presence of an activator site(s) between -52 and -185 was indicated by low activities conferred by the construct spanning this region.
Date of Original Version
1-1-2001
Rights Management
Deborah E. Britt
Citation Information
Diah, S. K., Padbury, J. F., Campbell, W. A., Britt, D., & Thompson, N. L. (2001). Molecular cloning of the rat TA1/LAT-1/CD98 light chain gene promoter. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression, 1518(3), 267-270. https://doi.org/10.1016/s0167-4781(01)00202-0