Skip to main content
Article
Spectroscopic Exploration of Mode of Binding of ctDNA with 3-Hydroxyflavone: A Contrast to the Mode of Binding with Flavonoids Having Additional Hydroxyl Groups
J. Phys. Chem. B, (2011)
  • Barnali Jana, Jadavpur University
  • Sudipta Senapati, Jadavpur University
  • Debanjana Ghosh, Jadavpur University
  • Debosreeta Bose, Jadavpur University
  • Nitin Chattopadhyay, Jadavpur University
Abstract
Binding interaction of 3-hydroxyflavone (3HF), a bioactive flavonoid, with calf-thymus DNA (ctDNA) has been explored exploiting various experimental techniques. The dual fluorescence of 3HF resulting from the excited state intramolecular proton transfer (ESIPT) is modified remarkably upon binding with the biomacromolecule. The determined binding constant, fluorescence quenching experiment, circular dichroism (CD) study, comparative binding study with the known intercalative binder ethidium bromide and thermometric experiment relating to the helix melting of ctDNA confirm the groove binding of 3HF with the DNA. This is in contrast to two other members of the flavonoid group, namely, fisetin and quercetin, where the bindings are established to be intercalative. The structural difference of 3HF from the other two probes with respect to the absence/presence of the additional hydroxyl groups is ascribed to be responsible for the difference in the mode of binding.
Keywords
  • ctDNA,
  • 3-Hydroxyflavone,
  • Flavonoids
Disciplines
Publication Date
November 30, 2011
DOI
10.1021/jp2094824
Citation Information
Barnali Jana, Sudipta Senapati, Debanjana Ghosh, Debosreeta Bose, et al.. "Spectroscopic Exploration of Mode of Binding of ctDNA with 3-Hydroxyflavone: A Contrast to the Mode of Binding with Flavonoids Having Additional Hydroxyl Groups" J. Phys. Chem. B, Vol. 116 Iss. 1 (2011) p. 639 - 645
Available at: http://works.bepress.com/debanjana-ghosh/6/