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Studies of Triton X-165 - β-Cyclodextrin Interactions Using Both Extrinsic and Intrinsic Fluorescence
Journal of Colloid and Interface Science (2010)
  • Debanjana Ghosh, Georgia Southern University
Abstract
The interaction of β-cyclodextrin with the non-ionic micelle-forming surfactant Triton X-165 (TX-165) has been studied using steady state fluorescence and fluorescence anisotropy techniques. Both extrinsic and intrinsic fluorescence have been exploited for the purpose. Phenosafranin (PSF), a cationic phenazinium dye, has been used as the extrinsic probe while fluorescence of TX-165 has served as the intrinsic one. PSF shows discernible interactions with both TX-165 and β-CD. The experimental results reveal that the extent of interaction of PSF with TX-165 is greater than with β-CD. However, addition of β-CD to a micellar solution of TX-165 containing PSF leads to a disruption of the micelles whereby the fluorophore is released from the micellar environment to the bulk aqueous phase. It has been substantiated that an inclusion complex is formed between the non-ionic surfactant and the cyclodextrin. A 1:1 stoichiometry of the TX-165–β-CD inclusion complex has been proposed. Such a complexation between TX-165 and β-CD results in an inhibition in the micellization process of TX-165 leading to an enhancement in the apparent CMC value. The inferences are drawn from a series of experiments, viz., binding studies, determination of micropolarity, heavy-ion quenching studies and steady state fluorescence anisotropy experiments monitoring both extrinsic and intrinsic fluorescences.
Keywords
  • Triton X-165,
  • β-cyclodextrin,
  • Phenosafranin,
  • Intrinsic fluorescence,
  • Fluorescence anisotropy
Disciplines
Publication Date
July 15, 2010
DOI
10.1016/j.jcis.2010.03.048
Citation Information
Debanjana Ghosh. "Studies of Triton X-165 - β-Cyclodextrin Interactions Using Both Extrinsic and Intrinsic Fluorescence" Journal of Colloid and Interface Science Vol. 347 Iss. 2 (2010) p. 252 - 259
Available at: http://works.bepress.com/debanjana-ghosh/12/