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In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin
  • Paul W. Dyce, University of Guelph
  • Jinghe Liu, University of Guelph
  • Chandrakant Tayade, Queen’s University
  • Gerald M. Kidder, Schulich School of Medicine & Dentistry
  • Dean H. Betts, Schulich School of Medicine & Dentistry
  • Julang Li, University of Guelph
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We previously reported that fetal porcine skin-derived stem cells were capable of differentiation into oocyte-like cells (OLCs). Here we report that newborn mice skin-derived stem cells are also capable of differentiating into early OLCs. Using stem cells from mice that are transgenic for Oct4 germline distal enhancer-GFP, germ cells resulting from their differentiation are expected to be GFP+. After differentiation, some GFP+ OLCs reached 40-45 μM and expressed oocyte markers. Flow cytometric analysis revealed that ∼0.3% of the freshly isolated skin cells were GFP+. The GFP-positive cells increased to ∼7% after differentiation, suggesting that the GFP+ cells could be of in vivo origin, but are more likely induced upon being cultured in vitro. To study the in vivo germ cell potential of skin-derived cells, they were aggregated with newborn ovarian cells, and transplanted under the kidney capsule of ovariectomized mice. GFP+ oocytes were identified within a subpopulation of follicles in the resulting growth. Our finding that early oocytes can be differentiated from mice skin-derived cells in defined medium may offer a new in vitro model to study germ cell formation and oogenesis. © 2011 Dyce et al.

Citation Information
Paul W. Dyce, Jinghe Liu, Chandrakant Tayade, Gerald M. Kidder, et al.. "In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin" PLoS ONE Vol. 6 Iss. 5 (2011)
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