Skip to main content
Article
High Efficiency Separation of Microbial Aggregates Using Capillary Electrophoresis
FEMS Microbiology Letters
  • Georg Schulte
  • David J. Westenberg, Missouri University of Science and Technology
  • Jeffrey M. Schneiderheinze
  • Daniel W. Armstrong, Missouri University of Science and Technology
Abstract
Recent advances in the technique of capillary electrophoresis have demonstrated fast, highly efficient separation of mixtures of intact microbes. This paper describes the application of this technique for the separation of microbial aggregates of Micrococcus luteus, Saccharomyces cerevisiae, or Alcaligenes faecalis. The aggregates of these microbes were resolved into several highly efficient peaks with analysis times under 10 min and efficiencies approaching 1 000 000 plates m−1 in some cases. A reproducible relationship was found between the electrophoretic mobility and the aggregation number or size of the cluster under a given set of experimental conditions. Often, cellular aggregation was reversible with brief immersion in an ultrasound bath. This reversibility was confirmed by visual microscopy and electrophoretic data.
Department(s)
Biological Sciences
Second Department
Chemistry
Library of Congress Subject Headings
Yeast
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2000 John Wiley & Sons, All rights reserved.
Publication Date
8-1-2000
Disciplines
Citation Information
Georg Schulte, David J. Westenberg, Jeffrey M. Schneiderheinze and Daniel W. Armstrong. "High Efficiency Separation of Microbial Aggregates Using Capillary Electrophoresis" FEMS Microbiology Letters (2000)
Available at: http://works.bepress.com/david_westenberg/9/