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Genetic Relationship among Diabrotica Species (Coleoptera: Chrysomelidae) Based on rDNA and mtDNA Sequences
Faculty Publications: Department of Entomology
  • Allen L. Szalanski, University of Nebraska at Lincoln
  • Richard L. Roehrdanz, USDA-ARS, Red River Valley Agricultural Research Center Biosciences Research Laboratory, Fargo, ND
  • David B. Taylor, University of Nebraska at Lincoln
Date of this Version
Published in Florida Entomologist 83(3) September, 2000, pp. 262-267.

Corn rootworms of the genus Diabrotica (Coleoptera: Chrysomelidae) are the most serious pest of corn in midwestern United States. Despite their economic importance, phylogenetic relationships within the genus remain unclear. Phylogenetic analysis of five Diabrotica species and subspecies was undertaken using DNA sequences of the nuclear rDNA first internal transcribed spacer region (ITS1) and a portion of the mtDNA cytochrome oxidase I and II genes (COI/COII). Parsimony and maximum likelihood analysis indicated that southern corn rootworm is sister to banded cucumber beetle, whereas, northern corn rootworm forms a distinct clade with western and Mexican corn rootworm. ITS1 and COI/COII were found to be useful markers for determining phylogenetic relationships among diabroticites.

Gusanos de raíz de maíz del genero Diabrotica (Coleoptera: Chrysomelidae) son la plaga de mayor seriedad para el maíz en el medio oeste de los Estados Unidos. A pesar de su importancia económica, relaciones filogenéticas dentro del genero permanecen confusas. Análisis filogenético de cinco especies de Diabrotica y subespecies fueron llevadas a cabo usando secuencias de ADN del [rDNA] nuclear primer orden, región [spacer] (ITS1) y una porción del [mtDNA cytochrome oxidase] I y II, genes (COI/ COII). Parsimonia y [cladograms] junta-vecinos indicaron que el gusano de raíz de maíz sureño es hermano del escarabajo bandeado del pepino, mientras que los gusanos de raíz de maíz norteños forman un [clade] diferente de con los gusanos de raíz de maíz occidentales y Mexicanos. ITS1 y COI/COII resultaron ser marcadores útiles para determinar las relaciones filogenéticas entre diabroticitas.

Citation Information
Allen L. Szalanski, Richard L. Roehrdanz and David B. Taylor. "Genetic Relationship among Diabrotica Species (Coleoptera: Chrysomelidae) Based on rDNA and mtDNA Sequences" (2000)
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