Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute
Active Transport, Cell Nucleus; Animals; Cell Line; Cell Nucleus; Mice; Microscopy, Fluorescence; *Molecular Probe Techniques; Myoblasts; Streptavidin
Genome activity and nuclear metabolism clearly depend on accessibility, but it is not known whether and to what extent nuclear structures limit the mobility and access of individual molecules. We used fluorescently labeled streptavidin with a nuclear localization signal as an average-sized, inert protein to probe the nuclear environment. The protein was injected into the cytoplasm of mouse cells, and single molecules were tracked in the nucleus with high-speed fluorescence microscopy. We analyzed and compared the mobility of single streptavidin molecules in structurally and functionally distinct nuclear compartments of living cells. Our results indicated that all nuclear subcompartments were easily and similarly accessible for such an average-sized protein, and even condensed heterochromatin neither excluded single molecules nor impeded their passage. The only significant difference was a higher frequency of transient trappings in heterochromatin, which lasted only tens of milliseconds. The streptavidin molecules, however, did not accumulate in heterochromatin, suggesting comparatively less free volume. Interestingly, the nucleolus seemed to exclude streptavidin, as it did many other nuclear proteins, when visualized by conventional fluorescence microscopy. The tracking of single molecules, nonetheless, showed no evidence for repulsion at the border but relatively unimpeded passage through the nucleolus. These results clearly show that single-molecule tracking can provide novel insights into mobility of proteins in the nucleus that cannot be obtained by conventional fluorescence microscopy. Our results suggest that nuclear processes may not be regulated at the level of physical accessibility but rather by local concentration of reactants and availability of binding sites.
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Copyright 2008 by the Biophysical Society. This is an Open Access article distributed under the terms of the Creative Commons-Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/2.0/), which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI of Published Version
Grünwald D, Martin RM, Buschmann V, Bazett-Jones DP, Leonhardt H, Kubitscheck U, Cardoso MC. Probing intranuclear environments at the single-molecule level. Biophys J. 2008 Apr 1;94(7):2847-58. doi:10.1529/biophysj.107.115014. Link to article on publisher's site
David Grunwald, Robert M Martin, Volker Buschmann, David P Bazett-Jones, et al.. "Probing intranuclear environments at the single-molecule level" Vol. 94 Iss. 7 (2008) ISSN: 0006-3495 (Linking)
Available at: http://works.bepress.com/david_grunwald/20/