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Article
Direct observation of single protein molecules in aqueous solution
Biochemistry and Molecular Pharmacology Publications and Presentations
  • David Grunwald, University of Massachusetts Medical School
  • Andreas Hoekstra, Rheinische Friedrich-Wilhelms-Universitat
  • Thomas Dange, Rheinische Friedrich-Wilhelms-Universitat
  • Volker Buschmann, Max-Delbruck-Centrum
  • Ulrich Kubitscheck, Rheinische Friedrich-Wilhelms-Universitat
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute
Date
4-10-2006
Document Type
Article
Medical Subject Headings
Carbocyanines; Diffusion; Microscopy, Fluorescence; Microscopy, Video; Sensitivity and Specificity; Solutions; Streptavidin; Time Factors; Water
Abstract

Single molecule tracking (SMT) by fluorescence video microscopy has matured to be a well established method in the last few years. In this report we demonstrate the imaging and tracking of single protein molecules in a phosphate/Hepes buffer with high sensitivity at frame rates of 350 Hz.

Comments

Citation: Grünwald, D., Hoekstra, A., Dange, T., Buschmann, V. and Kubitscheck, U. (2006), Direct Observation of Single Protein Molecules in Aqueous Solution. ChemPhysChem, 7: 812–815. doi: 10.1002/cphc.200500632

At the time of publication, David Grünwald was not yet affiliated with the University of Massachusetts Medical School.

Related Resources
Link to Article in PubMed
PubMed ID
16528778
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Citation Information
David Grunwald, Andreas Hoekstra, Thomas Dange, Volker Buschmann, et al.. "Direct observation of single protein molecules in aqueous solution" Vol. 7 Iss. 4 (2006) ISSN: 1439-4235 (Linking)
Available at: http://works.bepress.com/david_grunwald/17/