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Direct observation of single protein molecules in aqueous solution
Biochemistry and Molecular Pharmacology Publications and Presentations
  • David Grunwald, University of Massachusetts Medical School
  • Andreas Hoekstra, Rheinische Friedrich-Wilhelms-Universitat
  • Thomas Dange, Rheinische Friedrich-Wilhelms-Universitat
  • Volker Buschmann, Max-Delbruck-Centrum
  • Ulrich Kubitscheck, Rheinische Friedrich-Wilhelms-Universitat
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute
Publication Date
Document Type
Carbocyanines; Diffusion; Microscopy, Fluorescence; Microscopy, Video; Sensitivity and Specificity; Solutions; Streptavidin; Time Factors; Water
Single molecule tracking (SMT) by fluorescence video microscopy has matured to be a well established method in the last few years. In this report we demonstrate the imaging and tracking of single protein molecules in a phosphate/Hepes buffer with high sensitivity at frame rates of 350 Hz.
DOI of Published Version

Grünwald, D., Hoekstra, A., Dange, T., Buschmann, V. and Kubitscheck, U. (2006), Direct Observation of Single Protein Molecules in Aqueous Solution. ChemPhysChem, 7: 812–815. doi: 10.1002/cphc.200500632


At the time of publication, David Grünwald was not yet affiliated with the University of Massachusetts Medical School.

Related Resources
Link to Article in PubMed
PubMed ID
Citation Information
David Grunwald, Andreas Hoekstra, Thomas Dange, Volker Buschmann, et al.. "Direct observation of single protein molecules in aqueous solution" Vol. 7 Iss. 4 (2006) ISSN: 1439-4235 (Linking)
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