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Cell biology of mRNA decay
Biochemistry and Molecular Pharmacology Publications and Presentations
  • David Grunwald, University of Massachusetts Medical School
  • Robert H. Singer, Albert Einstein College of Medicine
  • Kevin Czaplinski, Stony Brook University
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute
Publication Date
Document Type
Book Chapter
Animals; Cell Survival; Humans; In Situ Hybridization, Fluorescence; Microscopy; Proteins; RNA Stability; RNA, Messenger

Studying single mRNA molecules has added new dimensions to our understanding of gene expression and the life cycle of mRNA in cells. Advances in microscopes and detection technology have opened access to single molecule research to most researchers interested in molecular biology. Here we provide an overview technique for single molecule studies of RNA in either fixed samples or in living cells. As part of a volume on mRNA turnover, it is increasingly relevant, because many of the recent advances in studies of mRNA turnover have suggested that there is non-homogeneous distribution of turnover factors in the cell. For this reason, understanding of spatial relationships between mRNA and mRNA turnover factors should enrich our understanding of this process.

DOI of Published Version

Grünwald D, Singer RH, Czaplinski K. Cell biology of mRNA decay. Methods Enzymol. 2008;448:553-77. doi: 10.1016/S0076-6879(08)02627-X. Link to article on publisher's site


At the time of publication, David Grünwald was not yet affiliated with the University of Massachusetts Medical School.

Related Resources
Link to article in PubMed
PubMed ID
Citation Information
David Grunwald, Robert H. Singer and Kevin Czaplinski. "Cell biology of mRNA decay" Vol. 448 (2008) ISSN: 1557-7988
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