Stem cells differentiation occurs naturally in a 3D environment with cell response linked to structure. It is not clearly defined how stem cells respond to 3D environments. Graphene is a novel and promising material with conductive and thermal capabilities that make it a potential viable bioscaffold for 2D and 3D structures. The osteogenic differentiation capabilities of murine mesenchymal stem cells (mMSC) were tested with and without mechanical stimulation in a variety of environments: glass, graphene film, collagen gel, and graphene foam. Graphene foam has shown to be a biocompatible micro environment that promotes cell proliferation with pore sizes of approximately 300-500 um. This study examines osteogenesis of mMSC’s in osteogenic media in known 2D and 3D environments (glass and collagen gel) against their graphene analogs (film and foam) while comparing the low-intensity vibration (LIV) against a non-vibrated group. Completion of this study will facilitate future studies in testing the efficacy of electrical compared to mechanical stimulus.