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Article
Distribution of endosomal, lysosomal and MHC markers in a monocytic cell line infected with Chlamydia psittaci
Infection and Immunity
  • David M. Ojcius, Institut Pasteur
  • Raymond Hellio, Institut Pasteur
  • Alice Dautry-Varsat, Institut Pasteur
ORCiD
David M. Ojcius: 0000-0003-1461-4495
Document Type
Article
Publication Date
6-1-1997
Abstract
The intracellular fate of Chlamydia psittaci during infection of a monocytic cell line, THP1, was characterized. Cytochalasin D inhibited phagocytosis of latex beads but had no effect on infection by C. psittaci, and vacuoles expressed the transferrin receptor, suggesting accessibility to the endocytic pathway. Early Chlamydia-containing vacuoles expressed major histocompatibility complex (MHC) class I molecules, and most vacuoles fused with host cell lysosomes, since they expressed LAMP-1 and had acidic pHs. In cells prestimulated with gamma interferon, vacuoles also expressed MHC class II molecules, suggesting that the monocytes might effectively process Chlamydia-derived antigens for presentation by MHC class I and class II molecules.
Citation Information
David M. Ojcius, Raymond Hellio and Alice Dautry-Varsat. "Distribution of endosomal, lysosomal and MHC markers in a monocytic cell line infected with Chlamydia psittaci" Infection and Immunity Vol. 65 Iss. 6 (1997) p. 2437 - 2442 ISSN: 0019-9567
Available at: http://works.bepress.com/david-ojcius/88/