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Characterization of severe fever with thrombocytopenia syndrome in rural regions of Zhejiang, China
PLoS One
  • Lei Zhang, Zhejiang Provincial Center for Disease Control and Prevention
  • Ling Ye, Center for Disease Control and Prevention
  • David M. Ojcius, University of California, Merced
  • Xiuyu Lou, Zhejiang Provincial Center for Disease Control and Prevention
  • Chengwei Wang, Center for Disease Control and Prevention
  • Cen Feng, Zhejiang Provincial Center for Disease Control and Prevention
  • Yi Sun, Zhejiang Provincial Center for Disease Control and Prevention
  • Zhongfa Wang, Center for Disease Control and Prevention
  • Shibo Li, Zhoushan Hospital
  • Yanjun Zhang, Zhejiang Provincial Center for Disease Control and Prevention
ORCiD
David M. Ojcius: 0000-0003-1461-4495
Document Type
Article
DOI
10.1371/journal.pone.0111127
Publication Date
10-30-2014
Abstract
Severe fever with thrombocytopenia syndrome virus (SFTSV) infections have recently been found in rural regions of Zhejiang. A severe fever with thrombocytopenia syndrome (SFTS) surveillance and sero-epidemiological investigation was conducted in the districts with outbreaks. During the study period of 2011–2014, a total of 51 SFTSV infection cases were identified and the case fatality rate was 12% (6/51). Ninety two percent of the patients (47/51) were over 50 years of age, and 63% (32/51) of laboratory confirmed cases occurred from May to July. Nine percent (11/120) of the serum samples from local healthy people without symptoms were found to be positive for antibodies to the SFTS virus. SFTSV strains were isolated by culture using Vero, and the whole genomic sequences of two SFTSV strains (01 and Zhao) were sequenced and submitted to the GenBank. Homology analysis showed that the similarity of the target nucleocapsid gene from the SFTSV strains from different geographic areas was 94.2–100%. From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters. Only the SFTSV strains from the Zhejiang (Daishan) region of China and the Yamaguchi, Miyazakj regions of Japan, were clustered into lineage II, consistent with both of these regions being isolated areas with similar geographic features. Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies. This study confirmed that SFTSV has been circulating naturally and can cause a seasonal prevalence in Daishan, China. The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.
Comments
Article e111127
Citation Information
Lei Zhang, Ling Ye, David M. Ojcius, Xiuyu Lou, et al.. "Characterization of severe fever with thrombocytopenia syndrome in rural regions of Zhejiang, China" PLoS One Vol. 9 Iss. 10 (2014) p. 1 - 7 ISSN: 1932-6203
Available at: http://works.bepress.com/david-ojcius/55/