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Article
Characterization of a monoclonal antibody reacting with antigen-4 domain of gp900 in Cryptosporidium parvum invasive stages
Parasitology Research
  • Alain Bonnin, Hôpital du Bocage
  • David M. Ojcius, Institut Pasteur
  • Philippe Souque, Institut Pasteur
  • Debra A. Barnes, University of California, San Francisco General Hospital
  • Patricia S. Doyle, University of California, San Francisco General Hospital
  • Jiri Gut, University of California, San Francisco General Hospital
  • Richard G. Nelson, University of California, San Francisco General Hospital
  • Carolyn Petersen, University of California, San Francisco General Hospital
  • Jean-Francois Dubremetz, Institut Pasteur
ORCiD
David M. Ojcius: 0000-0003-1461-4495
Document Type
Article
DOI
10.1007/s004360100424
Publication Date
8-1-2001
Abstract
Cryptosporidium parvum (Protozoa, Apicomplexa) infects the apical surface of intestinal epithelial cells, where it grows and divides within a membrane-bound parasitophorous vacuole. gp900, an abundant glycoprotein of C. parvum merozoites and sporozoites, is localized in micronemes and at the surface of invasive stages and participates in the invasion process. Here, we describe a new monoclonal antibody (mAb) against gp900. As shown by immunofluorescence of excysted parasites and immunoelectron microscopy of infected tissues, the mAb reacted with micronemes present in the apical pole of invasive stages. In immunoprecipitation experiments, the mAb was shown to react with a high molecular weight antigen co-migrating with gp900. Finally, three reactive clones were selected upon screening of a C. parvum genomic expression library with the mAb; and sequencing of the insert from one of them showed a 596 bp sequence identical to the DNA region encoding a domain of gp900 identified as antigen 4.
Citation Information
Alain Bonnin, David M. Ojcius, Philippe Souque, Debra A. Barnes, et al.. "Characterization of a monoclonal antibody reacting with antigen-4 domain of gp900 in Cryptosporidium parvum invasive stages" Parasitology Research Vol. 87 Iss. 8 (2001) p. 589 - 592 ISSN: 0932-0113
Available at: http://works.bepress.com/david-ojcius/53/