The roles of virus-derived small RNAs (vsRNAs) have been studied in plants and insects. However, the generation and function of small RNAs from cytoplasmic RNA viruses in mammalian cells remain unexplored. This study describes four vsRNAs that were detected in enterovirus 71-infected cells using next-generation sequencing and northern blots. Viral infection produced substantial levels (>105 copy numbers per cell) of vsRNA1, one of the four vsRNAs. We also demonstrated that Dicer is involved in vsRNA1 generation in infected cells. vsRNA1 overexpression inhibited viral translation and internal ribosomal entry site (IRES) activity in infected cells. Conversely, blocking vsRNA1 enhanced viral yield and viral protein synthesis. We also present evidence that vsRNA1 targets stem-loop II of the viral 5′ untranslated region and inhibits the activity of the IRES through this sequence-specific targeting. Our study demonstrates the ability of a cytoplasmic RNA virus to generate functional vsRNA in mammalian cells. In addition, we also demonstrate a potential novel mechanism for a positive-stranded RNA virus to regulate viral translation: generating a vsRNA that targets the IRES.
Available at: http://works.bepress.com/david-ojcius/25/
Funding: Ministry of Science and Technology, Taiwan [100–3112-B182–002, NSC 101–2325-B-182–015, 100–2221-E-182–056-MY3]; Chang Gung Memorial Hospital [CMRPD1A0671, NERPD2A0651]. Funding for open access charge: Ministry of Science and Technology, Taiwan [NSC 101–2325-B-182–015].
Conflict of interest statement. None declared.
The authors wish it to be known that, in their opinion, the first three authors should be regarded as joint First Authors.