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Activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin
PLoS One
  • Tsung-Teng Huang, National Central University
  • Fu-Guo Liu, National Central University
  • Chia-Fong Wei, Chang Gung University
  • Chia-Chen Lu, Fu Jen Catholic University
  • Chang-Chieh Chen, Chang Gung University
  • Hung-Chi Lin, Chang Gung University
  • David M. Ojcius, University of California, Merced
  • Hsin-Chih Lai, Chang Gung University
ORCiD
David M. Ojcius: 0000-0003-1461-4495
Document Type
Article
DOI
10.1371/journal.pone.0018308
Publication Date
4-12-2011
Abstract
Osajin is a prenylated isoflavone showing antitumor activity in different tumor cell lines. The underlying mechanism of osajin-induced cancer cell death is not clearly understood. In the present study, the mechanisms of osajin-induced cell death of human nasopharyngeal carcinoma (NPC) cells were explored. Osajin was found to significantly induce apoptosis of NPC cells in a dose- and time-dependent manner. Multiple molecular effects were observed during osajin treatment including a significant loss of mitochondrial transmembrane potential, release of cytochrome c into the cytosol, enhanced expression of Fas ligand (FasL), suppression of glucose-regulated protein 78 kDa (GRP78), and activation of caspases-9, -8, -4 and -3. In addition, up-regulation of proapoptotic Bax protein and down-regulation of antiapoptotic Bcl-2 protein were also observed. Taken together, osajin induces apoptosis in human NPC cells through multiple apoptotic pathways, including the extrinsic death receptor pathway, and intrinsic pathways relying on mitochondria and endoplasmic reticulum stress. Thus, osajin could be developed as a new effective and chemopreventive compound for human NPC.
Comments
Article e18308
Citation Information
Tsung-Teng Huang, Fu-Guo Liu, Chia-Fong Wei, Chia-Chen Lu, et al.. "Activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin" PLoS One Vol. 6 Iss. 4 (2011) p. 1 - 11 ISSN: 1932-6203
Available at: http://works.bepress.com/david-ojcius/24/