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cis-Resveratrol produces anti-inflammatory effects by inhibiting canonical and non-canonical inflammasomes in macrophages
Innate Immunity
  • Tsung-Teng Huang, Chang Gung University
  • Hsin-Chih Lai, Chang Gung University
  • Young-Bin Chen
  • Lih-Geeng Chen
  • Yi-Hui Wu
  • Yun-Fei Kourilsky
  • Chia-Chen Lu, Fu Jen Catholic University
  • Chih-Jung Chang
  • Cheng-Yeu Wu
  • Jan Martel, Chang Gung University
  • David M. Ojcius, University of the Pacific
  • Kowit-Yu Chong
  • John Ding-E. Young, Chang Gung University
David M. Ojcius: 0000-0003-1461-4495
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Resveratrol, a natural phenolic compound found in red grapes and wine, exists as cis and trans isomers. Recent studies have shown that trans-resveratrol possesses anti-inflammatory, anti-oxidant, anti-carcinogenic, anti-tumor and immunomodulatory properties. However, it remains unclear whether cis-resveratrol may exhibit similar activities. The objective of the present study was to examine the effects of cis- and trans-resveratrol on the production of pro-inflammatory cytokines and mediators in human macrophages. We examined the possibility that cis- and trans-resveratrol may affect cytokine secretion by modulating inflammasomes, intracellular multi-protein complexes, the assembly of which leads to caspase-1 activation and secretion of active IL-1β by macrophages. Our results show that pre-treatment of macrophages with cis-resveratrol not only reduces pro-IL-1β production and IL-1β secretion, but also suppresses ATP-induced transcription and activation of caspase-1 and caspase-4. Notably, cis-resveratrol inhibits the expression of the purinergic receptor, P2X7R, and the endoplasmic reticulum stress marker, Glc-regulated protein 78, but also reduces reactive oxygen species production. Moreover, cis-resveratrol attenuates cyclooxygenase-2 expression and prostaglandin E2 production. cis-Resveratrol also decreases the phosphorylation of p38 MAPK and expression of the c-Jun protein. These results indicate that cis-resveratrol produces anti-inflammatory effects by inhibiting both the canonical and non-canonical inflammasomes, and associated pathways in human macrophages.
Citation Information
Tsung-Teng Huang, Hsin-Chih Lai, Young-Bin Chen, Lih-Geeng Chen, et al.. "cis-Resveratrol produces anti-inflammatory effects by inhibiting canonical and non-canonical inflammasomes in macrophages" Innate Immunity Vol. 20 Iss. 7 (2014) p. 735 - 750 ISSN: 1753-4259
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