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Article
Activation of NK cell cytotoxicity by the natural compound 2,3-butanediol
Journal of Leukocyte Biology
  • Hsin-Chih Lai, Chang Gung University
  • Chih-Jung Chang, Chang Gung University
  • Chun-Hung Yang, Chang Gung University
  • Ya-Jing Hsu, Chang Gung University
  • Chang-Chieh Chen, Chang Gung University
  • Chuan-Sheng Lin, Chang Gung University
  • Yu-Huan Tsai, Chang Gung University
  • Tsung-Teng Huang, Chang Gung University
  • David M. Ojcius, Chang Gung University
  • Ying-Huang Tsai, Chang Gung Memorial Hospital
  • Chia-Chen Lu, Fu Jen Catholic University
ORCiD
David M. Ojcius: 0000-0003-1461-4495
Document Type
Article
DOI
10.1189/jlb.0112024
Publication Date
10-1-2012
Abstract

The natural compound 2,3-BTD has diverse physiological effects in a range of organisms, including acting as a detoxifying product of liver alcohol metabolism in humans and ameliorating endotoxin-induced acute lung injury in rats. In this study, we reveal that 2,3-BTD enhances NK cell cytotoxic activity in human pNK cells and NK92 cells. Treatment of NK cells with 2,3-BTD increased perforin expression in a dose-dependent manner. This was accompanied by elevated JNK and ERK1/2 MAPK activities and enhanced expression of NKG2D/NCRs, upstream signaling molecules of the MAPK pathways. The 2,3-BTD effect was inhibited by pretreatment with inhibitors of JNK (SP) or ERK1/2 (PD) or by depleting NKG2D/NCRs or JNK1 or ERK2 with siRNA. These results indicate that 2,3-BTD activates NK cell cytotoxicity by NKG2D/NCR pathways and represent the first report of the 2,3-BTD effect on activation of innate immunity cells.

Citation Information
Hsin-Chih Lai, Chih-Jung Chang, Chun-Hung Yang, Ya-Jing Hsu, et al.. "Activation of NK cell cytotoxicity by the natural compound 2,3-butanediol" Journal of Leukocyte Biology Vol. 92 Iss. 4 (2012) p. 807 - 814 ISSN: 0741-5400
Available at: http://works.bepress.com/david-ojcius/23/