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Article
The medicinal fungus Antrodia cinnamomea suppresses inflammation by inhibiting the NLRP3 inflammasome
Journal of Ethnopharmacology
  • Tsung-Teng Huang, Chang Gung University
  • Sian-Pu Wu
  • Kowit-Yu Chong
  • David M. Ojcius, University of the Pacific
  • Yun-Fei Ko, Chang Gung Biotechnology Corporation
  • Yi-Hui Wu
  • Cheng-Yeu Wu
  • Chia-Chen Lu, Fu Jen Catholic University
  • Jan Martel, Chang Gung University
  • John Ding-E. Young, Chang Gung University
  • Hsin-Chih Lai, Chang Gung University
ORCiD
David M. Ojcius: 0000-0003-1461-4495
Document Type
Article
DOI
10.1016/j.jep.2014.04.053
Publication Date
8-8-2014
Abstract
Ethnopharmacological relevance: Antrodia cinnamomea – a medicinal fungus that is indigenous to Taiwan – has been used as a health tonic by aboriginal tribes and the Asian population. Recent studies indicate that Antrodia cinnamomea extracts exhibit hepato-protective, anti-hypertensive, anti-oxidative, anti-inflammatory, immuno-modulatory, and anti-cancer effects on cultured cells and laboratory animals. This study aims to explore the anti-inflammatory activity of an Antrodia cinnamomea ethanol extract (ACEE) and elucidate its underlying mechanisms of action using lipopolysaccharide (LPS)-primed, ATP-stimulated human THP-1 macrophages. Materials and methods: The effects of ACEE on cell viability were studied using the MTT assay. The expressions of genes, proteins, and pro-inflammatory cytokines were measured by quantitative real-time RT-PCR, Western blotting and ELISA, respectively. The ACEE was further investigated for its effects on reactive oxygen species (ROS) production using ROS detection kit. Results: Our results showed that ACEE significantly inhibits ATP-induced secretion of interleukin-1β (IL-1β), interleukin-18 (IL-18) and tumor necrosis factor-α (TNF-α) by LPS-primed macrophages. ACEE also suppresses the transcription and activation of caspase-1, which is responsible for the cleavage and activation of IL-1β and IL-18. Of note, ACEE not only reduces expression of the inflammasome component NLRP3 and the purinergic receptor P2X7R but also inhibits ATP-induced ROS production and caspase-1 activation. Furthermore, the anti-inflammatory properties of ACEE correlate with reduced activation of the MAPK and NF-κB pathways. Conclusion: The results of the present study indicate that Antrodia cinnamomea suppresses the secretion of IL-1β and IL-18 associated with inhibition of the NLRP3 inflammasome in macrophages. These findings suggest that ACEE may have therapeutic potential for the treatment of inflammatory diseases.
Citation Information
Tsung-Teng Huang, Sian-Pu Wu, Kowit-Yu Chong, David M. Ojcius, et al.. "The medicinal fungus Antrodia cinnamomea suppresses inflammation by inhibiting the NLRP3 inflammasome" Journal of Ethnopharmacology Vol. 155 Iss. 1 (2014) p. 154 - 164 ISSN: 0378-8741
Available at: http://works.bepress.com/david-ojcius/226/