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Article
Subcellular localization of perforin and serine esterase in lymphokine-activated killer (LAK) cells and cytotoxic T cells by immunogold labelling
Journal of Immunology
  • David M. Ojcius, University of the Pacific
  • L. M. Zheng
  • E. C. Sphicas
  • A. Zychilinsky
  • John Ding-E. Young
ORCiD
David M. Ojcius: 0000-0003-1461-4495
Document Type
Article
Publication Date
6-15-1991
Abstract
CTL, NK cells, and lymphokine-activated killer (LAK) cells are cytolytic lymphocytes known to produce a pore-forming protein, named perforin or cytolysin, that lyses target cells by forming large pores on the plasma membrane of the target cell. Other proteins besides perforin are found in the cytoplasmic granules of effector lymphocytes, and these include a family of serine esterases. Ultrastructural immunogold labeling studies with antibodies against perforin and a serine esterase (MTSP-1, also known as granzyme A and SE-1) show that all the granules of LAK cells and a CTL cell line contain perforin and serine esterase. For both LAK cells and CTL, perforin has been located mostly in the fine granular matrix of the granules, whereas gold particles corresponding to serine esterase have been found in both the matrix and the cap regions of the granules. Results from double immunogold labeling indicate that perforin and serine esterase colocalize to the same granules.
Citation Information
David M. Ojcius, L. M. Zheng, E. C. Sphicas, A. Zychilinsky, et al.. "Subcellular localization of perforin and serine esterase in lymphokine-activated killer (LAK) cells and cytotoxic T cells by immunogold labelling" Journal of Immunology Vol. 146 Iss. 12 (1991) p. 4427 - 4432 ISSN: 0022-1767
Available at: http://works.bepress.com/david-ojcius/215/