Skip to main content
Article
Pretreatment with a heat-killed probiotic modulates monocyte chemoattractant protein-1 and reduces the pathogenicity of influenza and enterovirus 71 infections
Mucosal Immunology
  • M. F. Chen, Chang Gung University
  • K. F. Weng, Chang Gung University
  • S. Y. Huang, Chang Gung University
  • Y. C. Liu, Chang Gung University
  • S. N. Tseng, Chang Gung University
  • David M. Ojcius, University of the Pacific
  • S. R. Shih, Chang Gung University
ORCiD
David M. Ojcius: 0000-0003-1461-4495
Document Type
Article
DOI
10.1038/mi.2016.31
Publication Date
4-13-2016
Abstract
It has been proposed that inactivated probiotics may modulate the host immune system and contribute to mitigation of viral infections. This study demonstrated that administration of heat-killed Enterococcus faecalis, a widely used probiotic, can protect host animals against viral infections. The influenza-mediated morbidity and lung inflammation in E. faecalis-treated mice decreased significantly compared with those of the control mice. Furthermore, we found that the protection is associated with production of monocyte chemoattractant protein-1 (MCP-1). The intratracheal injection of a recombinant mouse MCP-1 protein abrogated the antiviral effects elicited by pretreatment with E. faecalis. CC chemokine receptor 2 (CCR2) is a receptor for MCP-1, and the intraperitoneal administration of a CCR2 antagonist effectively inhibited viral pathogenicity. The reduced pathogenicity was also observed in CCR2-deficient mice. Finally, E. faecalis significantly attenuated neuropathogenicity induced by another RNA virus, enterovirus 71. This study demonstrates that killed probiotics can reduce viral disease severity and identify that the MCP-1 pathway might act as a key mediator in the improved antiviral immune response. Our findings suggest that MCP-1 and its related signaling pathway can serve as critical therapeutic targets for development of new antiviral strategies.
Citation Information
M. F. Chen, K. F. Weng, S. Y. Huang, Y. C. Liu, et al.. "Pretreatment with a heat-killed probiotic modulates monocyte chemoattractant protein-1 and reduces the pathogenicity of influenza and enterovirus 71 infections" Mucosal Immunology Vol. 10 (2016) p. 215 - 227 ISSN: 1933-0219
Available at: http://works.bepress.com/david-ojcius/187/