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Article
A mouse model of human adaptive immune functions: HLA-A2.1-HLA-DR1-transgenic H-2 class I-/class II-knockout mice
European Journal of Immunology
  • Anthony Pajot
  • Marie-Louise Michel, Institut Pasteur
  • Nicolas Fazilleau
  • Veronique Pancre
  • Claude Auriault
  • David M. Ojcius, University of the Pacific
  • Francois A. Lemonnier
  • Yu-Chun Lone, Institut Pasteur
ORCiD
David M. Ojcius: 0000-0003-1461-4495
Document Type
Article
DOI
10.1002/eji.200425463
Publication Date
11-1-2004
Abstract

HLA-A2.1-/HLA-DR1-transgenic H-2 class I-/class II-knockout mice were created and their immunological potential evaluated in response to hepatitis B DNA vaccine. Every single immunized mouse developed hepatitis B virus-specific antibodies, HLA-DR1-restricted helper, and HLA-A2.1-restricted cytolytic T cell responses directed at the same immunodominant epitopes as those identified in naturally infected or vaccinated humans. These mice were specifically protected against a hepatitis B-recombinant vaccinia virus infection with a 10,000-fold or more reduction of the virus load at day 4 post-challenge. These mice represent a unique in vivo experimental model for human immune function studies without any interference with mouse MHC response which dwarfed the prediction of human responses. Furthermore, they enable the complete monitoring of immune adaptative responses for preclinical screening of candidate vaccines.

Citation Information
Anthony Pajot, Marie-Louise Michel, Nicolas Fazilleau, Veronique Pancre, et al.. "A mouse model of human adaptive immune functions: HLA-A2.1-HLA-DR1-transgenic H-2 class I-/class II-knockout mice" European Journal of Immunology Vol. 34 Iss. 11 (2004) p. 3060 - 3069 ISSN: 0014-2980
Available at: http://works.bepress.com/david-ojcius/12/