Identification of novel HLA-DR1-restricted epitopes from the hepatitis B virus envelope protein in mice expressing HLA-DR1 and vaccinated human subjectsMicrobes and Infection
ORCiDDavid M. Ojcius: 0000-0003-1461-4495
AbstractHelper T lymphocytes that control CD8+ T-cell and antibody responses are key elements for the resolution of infection by the hepatitis B virus and for the development of effective immunological memory after hepatitis B vaccination. We have used H-2 class II-deficient mice that express the human MHC class II molecule, HLA-DR1, to identify novel hepatitis B virus envelope-derived T helper epitopes. We confirmed the immunogenicity of a previously described HLA-DR1-restricted epitope, and identified three novel epitopes. CD4+ T-cell immune responses against these epitopes were detected in peripheral blood mononuclear cells from HLA-DR1+ individuals vaccinated against hepatitis B. We showed that subjects receiving the currently available hepatitis B vaccines do not develop cross-reactive T helper responses against one of the novel epitopes which are structurally variable between different hepatitis B virus subtypes. These findings highlight the need for developing vaccines against a wider range of viral subtypes, and establish humanized mice as a convenient tool for identifying new immunogenic epitopes from pathogens.
Citation InformationAnthony Pajot, Marie-Louise Michel, Maryline Mancini-Bourgine, Marie-Noelle Ungeheuer, et al.. "Identification of novel HLA-DR1-restricted epitopes from the hepatitis B virus envelope protein in mice expressing HLA-DR1 and vaccinated human subjects" Microbes and Infection Vol. 8 Iss. 12--13 (2006) p. 2783 - 2790 ISSN: 1286-4579
Available at: http://works.bepress.com/david-ojcius/115/