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Ganoderma lucidum stimulates NK cell cytotoxicity by inducing NKG2D/NCR activation and secretion of perforin and granulysin
Innate Immunity
  • Chih-Jung Chang, Chang Gung University
  • Yi-Yuan M. Chen, Chang Gung University
  • Chia-Chen Lu, Fu Jen Catholic University
  • Chuan-Sheng Lin, Chang Gung University
  • Jan Martel, Chang Gung University
  • Sheng-Hui Tsai, National Yang-Ming University
  • Yun-Fei Ko, Chang Gung Biotechnology Corporation
  • Tsung-Teng Huang, Chang Gung University
  • David M. Ojcius, Chang Gung University
  • John Ding-E. Young, Chang Gung University
  • Hsin-Chih Lai, Chang Gung University
ORCiD
David M. Ojcius: 0000-0003-1461-4495
Document Type
Article
DOI
10.1177/1753425913491789
Publication Date
6-26-2013
Abstract
Ganoderma lucidum (G. lucidum) is a medicinal mushroom long used in Asia as a folk remedy to promote health and longevity. Recent studies indicate that G. lucidum activates NK cells, but the molecular mechanism underlying this effect has not been studied so far. To address this question, we prepared a water extract of G. lucidum and examined its effect on NK cells. We observed that G. lucidum treatment increases NK cell cytotoxicity by stimulating secretion of perforin and granulysin. The mechanism of activation involves an increased expression of NKG2D and natural cytotoxicity receptors (NCRs), as well as increased phosphorylation of intracellular MAPKs. Our results indicate that G. lucidum induces NK cell cytotoxicity against various cancer cell lines by activating NKG2D/NCR receptors and MAPK signaling pathways, which together culminate in exocytosis of perforin and granulysin. These observations provide a cellular and molecular mechanism to account for the reported anticancer effects of G. lucidum extracts in humans.
Citation Information
Chih-Jung Chang, Yi-Yuan M. Chen, Chia-Chen Lu, Chuan-Sheng Lin, et al.. "Ganoderma lucidum stimulates NK cell cytotoxicity by inducing NKG2D/NCR activation and secretion of perforin and granulysin" Innate Immunity Vol. 20 Iss. 3 (2013) p. 301 - 311 ISSN: 1753-4259
Available at: http://works.bepress.com/david-ojcius/105/