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Article
Microsatellite Primers for the Atlantic Coastal Killifish, Fundulus Heteroclitus, with Applicability to Related Fundulus Species
Molecular Ecology Notes
  • Stephanie M. Adams
  • Marjorie F. Oleksiak
  • David D. Duvernell, Missouri University of Science and Technology
Abstract

The mummichog (Fundulus heteroclitus), a common Atlantic coastal killifish, is a model vertebrate species for the study of molecular genetic variation in natural populations and of environmental toxicology. We report the development of a set of 20 microsatellite loci in this species. Average expected heterozygosity across all loci was 0.84 (range: 0.60-0.97), revealing a high level of variability at most loci. A survey of seven additional Fundulus species yielded one or two robust amplification products in over half (63%) of the species-primer combinations tested. Therefore, many of these loci will also prove useful in studies of other members of the genus Fundulus.

Department(s)
Biological Sciences
Keywords and Phrases
  • Cyprinodontiformes,
  • Fundulus,
  • Fundulus catenatus,
  • Fundulus cingulatus,
  • Fundulus confluentus,
  • Fundulus heteroclitus,
  • Fundulus majalis,
  • Fundulus olivaceus,
  • Fundulus seminolis,
  • Fundulus similis,
  • Vertebrata
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2005 Blackwell Publishing, All rights reserved.
Publication Date
2-1-2005
Publication Date
01 Feb 2005
Disciplines
Citation Information
Stephanie M. Adams, Marjorie F. Oleksiak and David D. Duvernell. "Microsatellite Primers for the Atlantic Coastal Killifish, Fundulus Heteroclitus, with Applicability to Related Fundulus Species" Molecular Ecology Notes Vol. 5 Iss. 2 (2005) p. 275 - 277 ISSN: 1471-8278
Available at: http://works.bepress.com/david-duvernell/23/