Article
Isolation and Characterization of Microsatellite Loci for the Blackstripe Topminnow Fundulus Notatus and Their Variability in Two Closely Related Species
Journal of Fish Biology
Abstract
A total of 17 polymorphic microsatellite loci were isolated from the blackstripe topminnow Fundulus notatus. In a sample of 29 individuals, these loci were found to possess two to 19 alleles with expected heterozygosity values ranging from 0·212 to 0·919 and all but one of the loci conformed to Hardy–Weinberg equilibrium expectations. Many of these loci were polymorphic in the closely related species Fundulus olivaceus and Fundulus euryzonus providing a set of markers that should prove useful in future ecological and evolutionary studies of members of this species complex.
Department(s)
Biological Sciences
Keywords and Phrases
- Fundulidae,
- Fundulus notatus,
- microsatellite DNA,
- allele,
- animal,
- Cypriniformes,
- DNA sequence,
- gene locus,
- genetics,
- Alleles,
- Animals,
- Cyprinidae,
- Genetic Loci,
- Microsatellite Repeats,
- Sequence Analysis,
- DNA,
- Cross-species amplification,
- Fundulidae,
- Genetic marker,
- Population genetics,
- Primer sequence
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2014 Blackwell Publishing Ltd, All rights reserved.
Publication Date
8-1-2014
Publication Date
01 Aug 2014
Disciplines
Citation Information
Kevin Andrew Feldheim, Brian R. Kreiser, Bjorn Christian Schmidt, David D. Duvernell, et al.. "Isolation and Characterization of Microsatellite Loci for the Blackstripe Topminnow Fundulus Notatus and Their Variability in Two Closely Related Species" Journal of Fish Biology Vol. 85 Iss. 5 (2014) p. 1726 - 1732 ISSN: 0022-1112 Available at: http://works.bepress.com/david-duvernell/15/