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Article
Using Microbial Genomics to Evaluate the Effectiveness of Silver to Prevent Biofilm Formation
Water Science and Technology
  • Mi Y. Wu
  • K. Suryanarayanan
  • Ooij Wim Van
  • Daniel B. Oerther, Missouri University of Science and Technology
Abstract
Increasingly, monovalent silver cations are being considered as an alternative biocide in water distribution systems. The objective of this study was to understand how bacteria respond when challenged with silver. The approach used included cultivation of Escherichia coli in planktonic and sessile phases, exposing biomass to a solution of silver nitrate and evaluating genetic responses using commercial Affymetrix microarrays. Experimental results showed that lower pH enhanced silver toxicity in a dose-dependent manner. Sessile biomass demonstrated resistance to silver, and the microarray results indicated that the genetic mechanism for silver resistance was similar to the mechanism for copper resistance including upregulation of efflux pumps as well as upregulation of metal oxidoreductases. The gene, copA, a P-type ATPase efflux flux, was upregulated in response to silver exposure, and the gene of CusCFBA, a Cu(I) efflux pump, was also upregulated. The gene of CueO, a robust cuprous oxidase, was also upregulated and may have reduced silver toxicity through oxidation of silver ions. This study is significant because it provides baseline data to understand the genetic response of bacteria to silver biocides.
Department(s)
Civil, Architectural and Environmental Engineering
Keywords and Phrases
  • Bacteria,
  • Biofilms,
  • Cultivation,
  • Escherichia coli,
  • Microarrays,
  • Silver,
  • Water analysis,
  • Water distribution systems,
  • Biofilm annular reactor,
  • Silver rsistance,
  • Silver toxicity,
  • Bacteriology,
  • adenosine triphosphatase,
  • biocide,
  • copper,
  • copper exporting adenosine triphosphatase,
  • oxidoreductase,
  • biofilm,
  • biomass,
  • bioreactor,
  • coliform bacterium,
  • genomics,
  • oxidation,
  • plankton,
  • sessile species,
  • toxicity,
  • clinical effectiveness,
  • conference paper,
  • DNA microarray,
  • effusion,
  • gene expression,
  • microbial genetics,
  • molecular cloning,
  • nonhuman,
  • pH,
  • photooxidation,
  • RNA extraction,
  • species cultivation,
  • statistical analysis,
  • upregulation,
  • Bioreactors,
  • Disinfectants,
  • DNA,
  • Bacterial,
  • Escherichia coli Proteins,
  • Gene Expression Profiling,
  • Oligonucleotide Array Sequence Analysis,
  • Silver Nitrate,
  • Microarray,
  • Silver resistance
International Standard Book Number (ISBN)
1843395932; 9781843395935
Document Type
Article - Conference proceedings
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2007 IWA Publishing, All rights reserved.
Publication Date
4-1-2007
Citation Information
Mi Y. Wu, K. Suryanarayanan, Ooij Wim Van and Daniel B. Oerther. "Using Microbial Genomics to Evaluate the Effectiveness of Silver to Prevent Biofilm Formation" Water Science and Technology Vol. 55 Iss. 8-9 (2007) p. 413 - 419 ISSN: 02731223
Available at: http://works.bepress.com/daniel_oerther/94/