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Article
Fabrication of Culture-Based Biochips for Detecting Microorganisms in Environmental Samples
Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology (2002, Houston, TX)
  • Hima B. Eluru
  • Gaoshan Jing
  • Amy L. Polaczyk
  • Brian K. Kinkle
  • Daniel B. Oerther, Missouri University of Science and Technology
  • Ian Papautsky
Abstract

In this paper, we describe a culture-based biochip for detecting microorganisms in environmental samples in an improved, timesaving, and cost-efficient manner. The biochips use the paraffinophillic nature of microorganisms to rapidly detect and differentiate them in sewage samples collected from wastewater treatment plants. A new method of depositing paraffin was developed to fabricate the biochips. The developed technique uses butanol solvent to keep paraffin liquid during spin-coating and thick photoresist for patterning. Using this approach, paraffin layers of 5-70 µm have been successfully fabricated and patterned. These biochips were used to demonstrate rapid, 5-min detection of microorganisms responsible for reactor foaming in wastewater treatment.

Meeting Name
IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) (2002: Oct. 23-26, Houston, TX)
Department(s)
Civil, Architectural and Environmental Engineering
Keywords and Phrases
  • Activated sludge process,
  • Biotechnology,
  • Cell culture,
  • Foams,
  • Molecular biology,
  • Paraffins,
  • Photoresists,
  • Sewage analysis,
  • Spin coating,
  • Biochips,
  • Mycobacteria,
  • Nocardiafoam,
  • Paraffinophilic nature,
  • Microorganisms
Document Type
Article - Conference proceedings
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2002 Institute of Electrical and Electronics Engineers Inc. (IEEE) , All rights reserved.
Publication Date
10-1-2002
Citation Information
Hima B. Eluru, Gaoshan Jing, Amy L. Polaczyk, Brian K. Kinkle, et al.. "Fabrication of Culture-Based Biochips for Detecting Microorganisms in Environmental Samples" Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology (2002, Houston, TX) Vol. 2 (2002) p. 1688 - 1689 ISSN: 05891019
Available at: http://works.bepress.com/daniel_oerther/57/