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Effect of Changing VOC Influent Composition on the Microbial Community Structure of TBABs
Water, Air, and Soil Pollution: Focus
  • Zhangli Cai
  • George A. Sorial
  • Kai Zhang
  • Pascal E. Saikaly
  • Maher M. Zein
  • Daniel B. Oerther, Missouri University of Science and Technology

Microbial communities in trickle bed air biofilters (TBABs) were evaluated under conditions of interchanging the feed volatile organic compounds (VOCs) and VOC mixtures. Three independent TBABs (Biofilter "A," "B," and "C") were run under interchanging VOCs conditions with different initial VOCs. Two aromatic compounds (toluene and styrene) and two oxygenated compounds (methyl ethyl ketone (MEK) and methyl isobutyl ketone (MIBK)) were interchanged as single solutes. Two other TBABs "D" and "E" were run for two VOC mixtures. Biofilter "D" had a VOC mixture with equal molar ratio of the four components and Biofilter "E" received a VOC mixture with its composition based on EPA 2003 emission report. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes was used to assess the microbial richness in TBABs for treating the VOC mixtures and the impact of interchanging VOCs on the bacterial community structure in the biofilters. The results from DGGE indicated that the microbial community structure in the biofilter was different after each interchange of VOCs. Some bands of microbial species faded and some bands were strengthened. For the two TBABs treating VOC mixtures, the microbial species did not show significant difference, but the richness among these species was different from each other.

Civil, Architectural and Environmental Engineering
Keywords and Phrases
  • biofiltration,
  • chemical composition,
  • community structure,
  • electrokinesis,
  • microbial community,
  • species richness,
  • volatile organic compound,
  • Bacteria (microorganisms),
  • 16S rRNA,
  • Biofiltration,
  • DGGE,
  • Trickle bed air biofilter,
  • VOCs
Document Type
Article - Journal
Document Version
File Type
© 2008 Springer Netherlands, All rights reserved.
Publication Date
Citation Information
Zhangli Cai, George A. Sorial, Kai Zhang, Pascal E. Saikaly, et al.. "Effect of Changing VOC Influent Composition on the Microbial Community Structure of TBABs" Water, Air, and Soil Pollution: Focus Vol. 8 Iss. 3-4 (2008) p. 311 - 321 ISSN: 1567-7230
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