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Article
Developing Culture-Based Biochips for Detecting Microorganisms in Wastewater Treatment
Proceedings of the 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology (2002, Madison, WI)
  • Hima B. Eluru
  • Amy L. Polaczyk
  • Rajeev Chhabra
  • Brian K. Kinkle
  • Daniel B. Oerther, Missouri University of Science and Technology
  • Ian Papautsky
Abstract
In this paper, we describe a culture-based biochip for detecting microorganisms in environmental samples in an improved, timesaving, and cost-efficient manner. The biochips use the paraffinophilic nature of microorganisms to rapidly detect and differentiate them in sewage samples collected from wastewater treatment plants. A new method of depositing paraffin was developed in order to fabricate such biochips. The developed technique uses butanol solvent to keep paraffin liquid during deposition by spin-coating and thick photoresist for patterning. Using this approach, 18 mm glass biochips with paraffin layers of 5-70 µm have been successfully fabricated. These biochips were used to demonstrate rapid, 5-min detection of microorganisms responsible for reactor foaming in wastewater treatment.
Meeting Name
2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology (2002: May 2-4, Madison, WI)
Department(s)
Civil, Architectural and Environmental Engineering
Keywords and Phrases
  • Bioassay,
  • Biochips,
  • Microarrays,
  • Microorganisms,
  • Photoresists,
  • Plants (botany),
  • Reclamation,
  • Sewage,
  • Sewage treatment plants,
  • Cost-efficient,
  • Environmental sample,
  • Glass biochips,
  • mycobacteria,
  • Thick photoresists,
  • Wastewater treatment plants,
  • Wastewater treatment,
  • mycobacteria,
  • paraffin
International Standard Book Number (ISBN)
0780374800; 9780780374805
Document Type
Article - Conference proceedings
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2002 Institute of Electrical and Electronics Engineers Inc. (IEEE) , All rights reserved.
Publication Date
5-1-2002
Citation Information
Hima B. Eluru, Amy L. Polaczyk, Rajeev Chhabra, Brian K. Kinkle, et al.. "Developing Culture-Based Biochips for Detecting Microorganisms in Wastewater Treatment" Proceedings of the 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology (2002, Madison, WI) (2002) p. 152 - 155
Available at: http://works.bepress.com/daniel_oerther/39/