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Culture-Based Biochip for Environmental Monitoring
Proceedings of SPIE - The International Society for Optical Engineering (2004, San Jose, CA)
  • Gaoshan Jing
  • Daniel B. Oerther, Missouri University of Science and Technology
  • Ian Papautsky

In this paper, we describe the development of a culture-based biochip for detecting mycobacteria in environmental samples. The biochips use the paraffinophilic nature of mycobacteria to rapidly detect and differentiate them from non-target microorganisms. New methods of depositing and patterning paraffin were developed to fabricate prototype biochips. Biochips were experimentally tested to demonstrate rapid detection of mycobacteria in environmental samples collected from a municipal sewage treatment plant. Our successful demonstration of the culture-based biochip technology presents an alternative approach for developing new technology to track microorganisms in complex environmental samples.

Meeting Name
Microfluidics, BioMEMS, and Medical Microsystems II (2004: Jan. 26-27, San Jose, CA)
Civil, Architectural and Environmental Engineering
Keywords and Phrases
  • Biochips,
  • Environmental monitoring,
  • Myobacteria,
  • Paraffin patterning,
  • Adhesives,
  • Bacteria,
  • Biomass,
  • Cell culture,
  • Curing,
  • Environmental engineering,
  • Fluorescence,
  • Health care,
  • Potable water,
  • RNA,
  • Sorption,
  • Spin coating,
  • Microprocessor chips
Document Type
Article - Conference proceedings
Document Version
File Type
© 2004 SPIE, All rights reserved.
Publication Date
Citation Information
Gaoshan Jing, Daniel B. Oerther and Ian Papautsky. "Culture-Based Biochip for Environmental Monitoring" Proceedings of SPIE - The International Society for Optical Engineering (2004, San Jose, CA) Vol. 5345 (2004) p. 68 - 77 ISSN: 0277-786X
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