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Article
Culture-Based MEMS Device to Track Gordonia in Activated Sludge
Environmental Science and Technology
  • Amy L. Polaczyk
  • Brian K. Kinkle
  • Ian Papautsky
  • Daniel B. Oerther, Missouri University of Science and Technology
Abstract

Previously, we reported on the use of microelectromechanical systems (MEMS) fabrication technologies to develop paraffin surfaces for miniaturization of culture-based detection and rapid quantification of Mycolata in environmental samples. In the current study, the novel culture-based biochip was calibrated with a broad range of pure cultures of Mycolata including Gordonia spp. isolated from activated sludge foam. The biochip successfully recovered Gordonia amarae spiked into a sample of mixed liquor collected from a municipal activated sludge system. Comparisons of these results with molecular biology-based assays including 16S rRNA-targeted fluorescence in situ hybridization (FISH) and antibody staining demonstrated that the biochip provides a more rapid and user-friendly platform for reliable identification and quantification of Mycolata in full-scale municipal activated sludge sewage treatment plants. The results of this work successfully demonstrate an alternative platform technology for inexpensive monitoring of environmental microorganisms using existing expertise by potential users in the area of bacterial cultivation.

Department(s)
Civil, Architectural and Environmental Engineering
Keywords and Phrases
  • Activated sludge process,
  • Antibodies,
  • Bacteria,
  • Cell culture,
  • Fluorescence,
  • Paraffins,
  • Sewage sludge,
  • Sewage treatment plants,
  • Activated sludge,
  • Gordonia,
  • Microelectromechanical systems (MEMS),
  • Mycolata,
  • Microelectromechanical devices,
  • RNA 16S,
  • alkane,
  • antibody,
  • bioassay,
  • cultivation,
  • electronic equipment,
  • microbial activity,
  • microorganism,
  • RNA,
  • sewage treatment,
  • bacterium culture,
  • bacterium identification,
  • bacterium isolation,
  • biochip,
  • device,
  • fluorescence in situ hybridization,
  • immunohistochemistry,
  • microelectromechanical system device,
  • molecular biology,
  • nonhuman,
  • quantitative analysis,
  • reliability,
  • sludge,
  • technology,
  • Calibration,
  • Gordonia Bacterium,
  • In Situ Hybridization,
  • Bacterial,
  • Ribosomal,
  • 16S,
  • Sewage,
  • Bacteria (microorganisms),
  • Gordonia amarae
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2006 American Chemical Society, All rights reserved.
Publication Date
4-1-2006
Citation Information
Amy L. Polaczyk, Brian K. Kinkle, Ian Papautsky and Daniel B. Oerther. "Culture-Based MEMS Device to Track Gordonia in Activated Sludge" Environmental Science and Technology Vol. 40 Iss. 7 (2006) p. 2269 - 2274 ISSN: 0013936X
Available at: http://works.bepress.com/daniel_oerther/36/