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16S Ribosomal RNA Tools Identify an Unexpected Predominance of Paenibacillus-Like Bacteria in an Industrial Activated Sludge System Suffering from Poor Biosolids Separation
Water Environment Research
  • Joyce M. Simpson
  • Peter George Stroot
  • Steve Gelman
  • Inan Beydilli
  • Sandra Dudley
  • Daniel B. Oerther, Missouri University of Science and Technology
Abstract
Molecular biology tools targeting 16S ribosomal RNA (16S rRNA) were used to identify a predominant bacterial population in a full-scale dairy wastewater activated sludge system suffering from poor biosolids separation. Gram and acridine orange staining indicated that viable, Gram-positive microorganisms were present in samples removed from the influent waste, stream and represented approximately 50% of total cell counts in samples removed from the mixed liquor. Subsequently, the "full-cycle 16S rRNA approach" showed that phylogenetic relatives of Paenibacillus spp., a low guanine-plus-cytosine percent DNA-content, Gram-positive microorganism, represented up to 30% of total 4,6-diamidino-2-phenylindole (DAPI)-stained cell counts in samples of mixed liquor. Although fluorescent in situ hybridizations with 16S rRNA-targeted oligonucleotide hybridization probes identified Paenibacillus-like spp. in samples removed from the influent waste stream, their abundance was less than 10% of total stained cell counts. Results of this study suggest that Paenibacillus-like spp. were present in low abundance in the influent waste stream, increased in relative abundance within the treatment system, and should be examined further as a candidate bacterial population responsible for poor biosolids separation. This study demonstrates that the full-cycle 16S rRNA approach can be used to identify candidate bacterial populations that may be responsible for operational upsets in full-scale activated sludge systems without prior information from cultivation or microscopic analyses.
Department(s)
Civil, Architectural and Environmental Engineering
Keywords and Phrases
  • 16S ribosomal RNA,
  • Activated sludge,
  • Dairy food processing,
  • Paenibacillus spp.,
  • Bacteria,
  • Dyes,
  • Molecular biology,
  • RNA,
  • Wastewater,
  • Wastewater treatment,
  • Activated sludge process,
  • 4',6 diamidino 2 phenylindole,
  • acridine orange,
  • oligonucleotide,
  • RNA 16S,
  • abundance,
  • bacterium,
  • biosolid,
  • phylogenetics,
  • water treatment,
  • bacterium identification,
  • cell count,
  • dairying,
  • fluorescence in situ hybridization,
  • Gram staining,
  • nonhuman,
  • oligonucleotide probe,
  • Paenibacillus,
  • phylogeny,
  • priority journal,
  • RNA analysis,
  • waste water management,
  • Bacillaceae,
  • Dairy Products,
  • Equipment Failure,
  • Food Industry,
  • Industrial Waste,
  • Models,
  • Biological,
  • Bacterial,
  • Ribosomal,
  • 16S,
  • Ultrafiltration,
  • Waste Disposal,
  • Fluid,
  • Water Microbiology,
  • Bacteria (microorganisms),
  • Posibacteria
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2006 Water Environment Federation, All rights reserved.
Publication Date
8-1-2006
Citation Information
Joyce M. Simpson, Peter George Stroot, Steve Gelman, Inan Beydilli, et al.. "16S Ribosomal RNA Tools Identify an Unexpected Predominance of Paenibacillus-Like Bacteria in an Industrial Activated Sludge System Suffering from Poor Biosolids Separation" Water Environment Research Vol. 78 Iss. 8 (2006) p. 864 - 871 ISSN: 10614303
Available at: http://works.bepress.com/daniel_oerther/22/