Comparison of Sterculic Oil Induced Changes in the Gut Microbiota of Lean and Obese MiceFASB Journal
AbstractRecent obesity research has reported differences in intestinal microbiota between obese ob/ob mice and lean wild type mice suggesting a potential link between bacterial populations and metabolic health. Sterculic oil (SO) is a natural inhibitor of stearoyl-CoA desaturase-1, a potential therapeutic target for the treatment/attenuation of obesity and insulin resistance.The aim of this study was to investigate the response of the obese and lean gut microbiota to a SO supplemented diet. Five-week old male lean (WT) and obese (ob/ob) mice were fed either a control AIN-93G diet or an AIN-93G diet containing 0.5% SO for 9 weeks Barcoded pyrosequencing of the V4-V5 region of the 16S rRNA gene was employed to characterize cecal microbiota. Intestinal microbiota from WT mice fed a control diet were more diverse, although there were no significant differences in the proportions of major phyla from WT and ob/ob mice. Irrespective of genotype, SO was negatively correlated with the relative abundances of Actinobacteria, Bacilli and Erysipelotrichi. This shift was not associated with a change in body weight, however a statistically significant improvement in glucose tolerance and insulin sensitivity was observed in the ob/ob mice fed SO. In our study, diet had a greater impact on gut microbiota as compared to genotype and these changes were associated with an improved metabolic health.
Meeting NameExperimental Biology 2012 (2012: Apr. 21-25, San Diego, CA)
Department(s)Civil, Architectural and Environmental Engineering
Sponsor(s)Missouri University of Science and Technology
Electronic OCLC #41042869
Print OCLC #15231392
Document TypeArticle - Conference proceedings
Rights© 2012 Federation of American Society of Experimental Biology (FASEB), All rights reserved.
Citation InformationShreya Ghosh, James W. Perfield, Pascal E. Saikaly and Daniel B. Oerther. "Comparison of Sterculic Oil Induced Changes in the Gut Microbiota of Lean and Obese Mice" FASB Journal Vol. 26 Iss. 1 (2012) p. 830.2 ISSN: 0892-6638
Available at: http://works.bepress.com/daniel_oerther/11/