
Article
Controlled Gating of Lysenin Pores
Biophysical Chemistry
(2010)
Abstract
Lysenin forms unitary large conductance pores in artificial bilayer membranes containing sphingomyelin. A population of lysenin pores inserted into such a bilayer membrane exhibited a dynamic negative conductance region, as predicted by a simple two-state model for voltage-gated channels. The recorded I-V curves demonstrated that lysenin pores inserted into the bilayer are uniformly oriented. Additionally, the transition between the two-states was affected by changes in the monovalent ion concentration and pH, pointing towards an electrostatic interaction governing the gating mechanism.
Keywords
- Lysenin,
- Voltage gated pores,
- Negative conductance
Disciplines
- Biophysics and
- Physics
Publication Date
January, 2010
Citation Information
Daniel Fologea, Eric Krueger, Rachel Lee, Matt Naglak, et al.. "Controlled Gating of Lysenin Pores" Biophysical Chemistry Vol. 146 Iss. 1 (2010) Available at: http://works.bepress.com/daniel_fologea/3/