SelectedWorks - Daniel Fologea

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Selected Works of Daniel Fologea

Professor, Department of Physics

Dr. Daniel Fologea studied physics and biophysics the University of Bucharest in Romania, and biolectrochemistry at Université Joseph Fourier in Grenoble France. He earned a Ph.D. in Biophysics from the University of Bucharest. He came to Boise State University in 2011 as a member of the faculty of the Department of Physics. Dr. Fologea's main research goal is to uncover the functionality of pore forming proteins, to understand their role and transport abilities, and to make use of such properties in developing artificial carriers for controlled drug delivery. He has published his findings widely and has made more than 175 presentations internationally at physics, biophysics, and biology conferences.

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About Daniel Fologea

Dr. Daniel Fologea studied physics and biophysics the University of Bucharest in Romania, and biolectrochemistry at Université Joseph Fourier in Grenoble France. He earned a Ph.D. in Biophysics from the University of Bucharest. He came to Boise State University in 2011 as a member of the faculty of the Department of Physics. Dr. Fologea's main research goal is to uncover the functionality of pore forming proteins, to understand their role and transport abilities, and to make use of such properties in developing artificial carriers for controlled drug delivery. He has published his findings widely and has made more than 175 presentations internationally at physics, biophysics, and biology conferences.

Articles (26)

Modulation of Voltage-Gating and Hysteresis of Lysenin Channels by Cu2+ Ions (Andrew Bogard, Pangaea W. Finn, Aviana R. Smith, et al.) International Journal of Molecular Sciences (2023)

The intricate voltage regulation presented by lysenin channels reconstituted in artificial lipid membranes leads to a strong hysteresis in conductance, bistability, and memory. Prior investigations on lysenin channels indicate that the hysteresis is modulated by ...

Hypo-Osmotic Stress and Pore-Forming Toxins Adjust the Lipid Order in Sheep Red Blood Cell Membranes (Rose Whiting, Sevio Stanton, Maryna Kucheriava, et al.) Membranes (2023)

Lipid ordering in cell membranes has been increasingly recognized as an important factor in establishing and regulating a large variety of biological functions. Multiple investigations into lipid organization focused on assessing ordering from temperature-induced phase ...

Liposome Formulation for Tumor-Targeted Drug Delivery Using Radiation Therapy (Daniel Fologea) International Journal of Molecular Sciences (2022)

Targeted delivery of drugs or other therapeutic agents through internal or external triggers has been used to control and accelerate the release from liposomal carriers in a number of studies, but relatively few utilize energy ...

Experimental Investigations on the Conductance of Lipid Membranes Under Differential Hydrostatic Pressure (Rose Whiting, Pangaea W. Finn, Andrew Bogard, et al.) Membranes (2022)

The unassisted transport of inorganic ions through lipid membranes has become increasingly relevant to an expansive range of biological phenomena. Recent simulations indicate a strong influence of a lipid membrane’s curvature on its permeability, which ...

The Ionic Selectivity of Lysenin Channels in Open and Sub-Conducting States (Andrew Bogard, Pangaea W. Finn, Fulton McKinney, et al.) Membranes (2021)

The electrochemical gradients established across cell membranes are paramount for the execution of biological functions. Besides ion channels, other transporters, such as exogenous pore-forming toxins, may present ionic selectivity upon reconstitution in natural and artificial ...

Rapid Production and Purification of Dye-Loaded Liposomes by Electrodialysis-Driven Depletion (Gamid Abatchev, Andrew Bogard, Zoe Hutchinson, et al.) Membranes (2021)

Liposomes are spherical-shaped vesicles that enclose an aqueous milieu surrounded by bilayer or multilayer membranes formed by self-assembly of lipid molecules. They are intensively exploited as either model membranes for fundamental studies or as vehicles ...

Liposomes Prevent in vitro Hemolysis Induced by Streptolysin O and Lysenin (Marcelo Ayllon, Gamid Abatchev, Andrew Bogard, et al.) Membranes (2021)

The need for alternatives to antibiotics in the fight against infectious diseases has inspired scientists to focus on antivirulence factors instead of the microorganisms themselves. In this respect, prior work indicates that tiny, enclosed bilayer ...

Lysenin Channels as Sensors for Ions and Molecules (Andrew Bogard, Gamid Abatchev, Zoe Hutchinson, et al.) Sensors (2020)

Lysenin is a pore-forming protein extracted from the earthworm Eisenia fetida, which inserts large conductance pores in artificial and natural lipid membranes containing sphingomyelin. Its cytolytic and hemolytic activity is rather indicative of a pore-forming ...

Kinetic Exclusion Assay of Biomolecules by Aptamer Capture (Mark H. Smith and Daniel Fologea) Sensors (2020)

DNA aptamers are short nucleotide oligomers selected to bind a target ligand with affinity and specificity rivaling that of antibodies. These remarkable features recommend aptamers as candidates for analytical and therapeutic applications that traditionally use ...

Temporary Membrane Permeabilization via the Pore-Forming Toxin Lysenin (Nisha Shrestha, Christopher A. Thomas, Devon Richtsmeier, et al.) Toxins (2020)

Pore-forming toxins are alluring tools for delivering biologically-active, impermeable cargoes to intracellular environments by introducing large conductance pathways into cell membranes. However, the lack of regulation often leads to the dissipation of electrical and chemical ...

An Effective Electric Dipole Model for Voltage-Induced Gating Mechanism of Lysenin (Eric Krueger and Daniel Fologea) Scientific Reports (2019)

Lysenin is a pore-forming toxin, which self-inserts open channels into sphingomyelin containing membranes and is known to be voltage regulated. The mechanistic details of its voltage gating mechanism, however, remains elusive despite much recent efforts. ...

Defect Engineering of ZnO Nanoparticles for Bioimaging Applications (Josh E. Eixenberger, Katelyn Wada, Kongara M. Reddy, et al.) ACS Applied Materials & Interfaces (2019)

Many promising attributes of ZnO nanoparticles (nZnO) have led to their utilization in numerous electronic devices and biomedical technologies. nZnO fabrication methods can create a variety of intrinsic defects that modulate the properties of nZnO, ...

Insights into the Voltage Regulation Mechanism of the Pore-Forming Toxin Lysenin (Sheenah Lynn Bryant, Tyler Clark, Christopher Alex Thomas, et al.) Toxins (2018)

Lysenin, a pore forming toxin (PFT) extracted from Eisenia fetida, inserts voltage-regulated channels into artificial lipid membranes containing sphingomyelin. The voltage-induced gating leads to a strong static hysteresis in conductance, which endows lysenin with molecular ...

ZnO Nanoparticles Modulate the Ionic Transport and Voltage Regulation of Lysenin Nanochannels (Sheenah L. Bryant, Josh E. Eixenberger, Steven Rossland, et al.) Journal of Nanobiotechnology (2017)

Background: The insufficient understanding of unintended biological impacts from nanomaterials (NMs) represents a serious impediment to their use for scientific, technological, and medical applications. While previous studies have focused on understanding nanotoxicity effects mostly resulting ...

Modeling and Analysis of Intercalant Effects on Circular DNA Conformation (Eric Krueger, Angela Nicole Chang, Katie M. Yocham, et al.) ACS Nano (2016)

The large-scale conformation of DNA molecules plays a critical role in many basic elements of cellular functionality and viability. By targeting the structural properties of DNA, many cancer drugs, such as anthracyclines, effectively inhibit tumor ...

Intramembrane Congestion Effects on Lysenin Channel Voltage-Induced Gating (Eric Krueger, Sheenah Bryant, Nisha Shrestha, et al.) European Biophysics Journal (2016)

All cell membranes are packed with proteins. The ability to investigate the regulatory mechanisms of protein channels in experimental conditions mimicking their congested native environment is crucial for understanding the environmental physicochemical cues that may ...

A Model for the Hysteresis Observed in Gating of Lysenin Channels (Eric Krueger, Radwan Al Faouri, Daniel Fologea, et al.) Biophysical Chemistry (2013)

The pore-forming toxin lysenin self-inserts to form conductance channels in natural and artificial lipid membranes containing sphingomyelin. The inserted channels exhibit voltage regulation and hysteresis of the macroscopic current during the application of positive periodic ...

Radiation-Induced Galectin-1 by Endothelial Cells: A Promising Molecular Target for Preferential Drug Delivery to the Tumor Vasculature (Meenakshi Upreti, Azemat Jamshidi-Parsian, Scott Apana, et al.) Journal of Molecular Medicine (2013)

The present study reports on a new strategy for selective, radiation therapy-amplified drug delivery using an antiangiogenic 33-a.a., tumor vasculature-targeting ligand, anginex, to improve the therapeutic ratio for strategies developed against solid tumors. Our findings ...

Cationic Polymers Inhibit the Conductance of Lysenin Channels (Daniel Fologea, Eric Krueger, Steven Rossland, et al.) The Scientific World Journal (2013)

The pore-forming toxin lysenin self-assembles large and stable conductance channels in natural and artificial lipidmembranes.The lysenin channels exhibit unique regulation capabilities, which open unexplored possibilities to control the transport of ions and molecules through artificial ...

Bi-Stability, Hysteresis, and Memory of Voltage-Gated Lysenin Channels (Daniel Fologea, Eric Krueger, Yuriy I. Mazur, et al.) Biochimica et Biophysica Acta- Biomembranes (2011)

Lysenin, a 297 amino acid pore-forming protein extracted from the coelomic fluid of the earthworm E. foetida, inserts constitutively open large conductance channels in natural and artificial lipid membranes containing sphingomyelin. The inserted channels show ...

Potential Analytical Applications of Lysenin Channels for Detection of Multivalent Ions (Daniel Fologea, Redwan Al Faori, Eric Krueger, et al.) Analytical and Bioanalytical Chemistry (2011)

Transmembrane protein transporters possessing binding sites for ions, toxins, pharmaceutical drugs, and other molecules constitute excellent candidates for developing sensitive and selective biosensing devices. Their attractiveness for analytical purposes is enhanced by the intrinsic amplification ...

Multivalent Ions Control the Transport Through Lysenin Channels (Daniel Fologea, Eric Krueger, Redwan Al Faori, et al.) Biophysical Chemistry (2010)

We report the effect of different ions on the conducting properties of lysenin channels inserted into planar lipid bilayer membranes. Our observations indicated that multivalent ions inhibited the lysenin channels conductance in a concentration dependent ...

Controlled Gating of Lysenin Pores (Daniel Fologea, Eric Krueger, Rachel Lee, et al.) Biophysical Chemistry (2010)

Lysenin forms unitary large conductance pores in artificial bilayer membranes containing sphingomyelin. A population of lysenin pores inserted into such a bilayer membrane exhibited a dynamic negative conductance region, as predicted by a simple two-state ...

The Membrane-binding Motif of the Chloroplast Signal Recognition Particle Receptor (cpFtsY) Regulates GTPase Activity (Naomi J. Marty, Dakshinamurthy Rajalingam, Alicia D. Kight, et al.) Journal of Biological Chemistry (2009)

The chloroplast signal recognition particle (cpSRP) and its receptor (cpFtsY) function in thylakoid biogenesis to target integral membrane proteins to thylakoids. Unlike cytosolic SRP receptors in eukaryotes, cpFtsY partitions between thylakoid membranes and the soluble ...

DNA Conformation and Base Number Simultaneously Determined in a Nanopore (Daniel Fologea, Eric Brandin, James Uplinger, et al.) Electrophoresis (2007)

When dsDNA polymers containing identical number of base pairs were electrophoresed through a nanopore in a voltage biased silicon nitride membrane, the measured time integral of blocked ionic current (the event-charge-deficit, ecd, Fologea, D., Gershow, ...