Cortical Type 2 Astrocytes are not Dye Coupled nor do they Express the Major Gap Junction Genes found in the Central Nervous SystemGlia (1994)
AbstractThe O‐2A progenitor cell first described from the rat optic nerve is a bipotential precursor of oligodendrocytes and type 2 astrocytes. Each cell expresses specific markers that distinguish them as unique cell types. O‐2A progenitors cultured in high serum preferentially differntiate into type 2 astrocytes and when exposed to defined medium or low serum develop along the oligodendrocyte lineage. We analyzed the gap junction gene expression of type 2 astrocytes to determine if they are coupled to form a syncytium, like their type 1 astrocyte counterparts. Dye coupling experiments demonstrated that cortical type 2 astrocytes are not coupled, while type 1 astrocytes in the same culture dish are highly coupled. Immunocytochemistry revealed the presence of Cx43 in type 1 astrocytes but we could not detect Cx26, 32, or 43 protein in type 2 astrocytes. In situ hybridization did not detect mRNA for any of the three connexin genes in type 2 astrocytes. These data demonstrate that type 2 astrocytes do not express the major gap junction genes found in the central nervous system. The precise function of type 2 astrocytes is not known but the lack of gap junction genes expression suggests that their functions are different from the spatial buffering capacity of type 1 astrocytes.
- 0-2A progenitor,
- perinodal astrocytes,
- cell culture,
Citation InformationDaniel Belliveau and C. C.G. Naus. "Cortical Type 2 Astrocytes are not Dye Coupled nor do they Express the Major Gap Junction Genes found in the Central Nervous System" Glia Vol. 12 Iss. 1 (1994)
Available at: http://works.bepress.com/daniel_belliveau/17/