Lanosterol is a sterol derivative that plays a crucial role in the biosynthesis pathway of cholesterol. Recently, it has been found to reverse the formation of cataracts. The physicochemical properties of lanosterol are poorly understood. Pure lanosterol (>95%) was isolated from a crude product (54.6%) by a newly developed C18 reverse-phase high-performance liquid chromatography (HPLC) method. Purity and structure were confirmed by gas chromatography-mass spectrometry (GC-MS). Ultraviolet-visible spectroscopy shows lanosterol has a maximum absorption peak at 202 nm and the extinction coefficient calculated to be 38280 M-1 ∙cm-1 . The melting temperature and fusion enthalpy were determined to be 407.22 K and 15.37 kJ∙mol-1, respectively, by differential scanning calorimeter (DSC). The solubility of lanosterol was measured in methanol, ethanol, acetonitrile, acetone, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO) and ethyl acetate using a static equilibrium set up from 284.65 K to 321.65 K. The solubility of lanosterol increases with an increase in temperature. The solubility of lanosterol has a minimum of 0.11mol∙L-1 in acetonitrile at 291.6 K and a maximum of 1.27 mol∙L-1 in ethylene acetate at 320.8 K. The solubilities of lanosterol in selected solvents were correlated by the modified Apelblat equation, Wilson model, NRTL model and UNIQUAC model.