The primary targets for SARS-CoV infection are the epithelial cells in the respiratory and intestinal tract. The angiotensin-converting enzyme 2 (ACE-2) has been identified as a functional receptor for SARS-CoV. ACE-2 has been shown to be expressed at the apical domain of polarized Calu-3 cells. In this report, interferon alfacon 1 was examined for inhibitory activities against SARS-CoV on human lung carcinoma epithelial Calu-3 cell line and the other three African green monkey kidney epithelial cell lines. Interferon alfacon 1 demonstrated significant antiviral activity in neutral red uptake assay and virus yield reduction assay. The data might provide an important insight into the mechanism of pathogenesis of SARS-CoV allowing further development of antiviral therapies for treating SARS infections.
Article
Interferon alfacon 1 inhibits SARS-CoVinfection in human bronchial epithelial Calu-3 cells
Interferon alfacon 1 inhibits SARS-CoVinfection in human bronchial epithelial Calu-3 cells
Document Type
Article
Publication Date
1-1-2008
Disciplines
Abstract
Citation Information
Kumaki, Y., Day, C.W., Wandersee, M.K., Schow, B.P., Madsen, J.S., Grant, D., Roth, J.P.,
Smee, D.F., Blatt, L.M., and Barnard, D.L.* 2008. Interferon alfacon 1 inhibits SARS-CoV
infection in human bronchial epithelial Calu-3 cells. Biochem. Biophys. Res. Commun.
371:110-113. PMID: 18406349
Biochem. Biophys. Res. Commun. 371:110-113. PMID: 18406349