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Activation of bovine peripheral blood gd T cells for cell division and IFN-g production
Veterinary Immunology and Immunopathology (2002)
  • Cynthia Baldwin, University of Massachusetts - Amherst
  • T. Sathiyaseelan
  • B. Naiman
  • A.M. White
  • R. Brown
  • S. Blumerman
  • A. Rogers
  • S.J. Black
Abstract
Bovine peripheral blood gammadelta T cells have been evaluated for effector function (IFN-gamma production) and clonal expansion in a variety of systems including following activation by mitogens, IL-12, and stimulation, through the T cell receptor (TCR) with anti-CD3 monoclonal antibody (mAb), a cell-bound molecule and a soluble antigenic extract. To evaluate cell division, carboxyfluorescein succinimidyl ester (CFSE) loading of cells and flow cytometric analysis were used, while IFN-gamma production was evaluated by intracytoplasmic staining. It was found that bovine gammadelta T cells produced IFN-gamma and clonally expanded when stimulated through the TCR/CD3 complex by a cell-associated autologous molecule on monocyte, by bacterial components following in vivo sensitization of gammadelta T cells with a leptospira vaccine or by anti-CD3 mAb. In addition, gammadelta T cells were activated efficiently for effector function but not clonal expansion by culturing with IL-12. In contrast, stimulation by Con A or PMA/ionomycin induced efficient replication but only low level IFN-gamma production which was not enhanced by the presence of IL-12. In several systems the amount of IFN-gamma produced per cell by gammadelta T cells was less than that produced by CD4 T cells in the same cultures.
Disciplines
Publication Date
2002
Citation Information
Cynthia Baldwin, T. Sathiyaseelan, B. Naiman, A.M. White, et al.. "Activation of bovine peripheral blood gd T cells for cell division and IFN-g production" Veterinary Immunology and Immunopathology Vol. 87 (2002)
Available at: http://works.bepress.com/cynthia_baldwin/5/