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Article
Expression of the bovine high affinity IL-12 receptor b2
Veterinary Immunology and Immunopathology (2002)
  • A.M. White
  • S. Blumerman
  • B. Naiman
  • Cynthia Baldwin, University of Massachusetts - Amherst
Abstract
Four fragments of the bovine IL-12 receptor beta2 were sequenced following generation by reverse transcriptase polymerase chain reaction (RT-PCR) amplification of RNA from mitogen-activated bovine peripheral blood mononuclear cells (PBMC). Primers were based on sequences within regions of the human IL-12Rbeta2 gene that displayed high levels of similarity with the mouse IL-12Rbeta2 gene sequence. The amplified bovine IL-12Rbeta2 fragments had 82-87% similarity at the nucleotide level with human IL-12Rbeta2 and 70-88% similarity at the predicted amino acid level. Bovine IL-12Rbeta2 gene expression was induced following culture of PBMC with Concanavalin A (Con A), with immobilized monoclonal antibody to CD3 or with human recombinant IL-12 p70 and correlated with interferon-gamma (IFN-gamma) production. Expression of bovine IL-12Rbeta2 by PBMC was detected by 2h of culture with Con A and sustained for at least 5 days when cultured with rHuIL-12. Expression, however, did not require cellular proliferation since IL-12 did not induce proliferation, although both Con A and anti-CD3 monoclonal antibody did do so. Addition of rHuIL-10 inhibited IFN-gamma production without abrogating bovine IL-12Rbeta2 gene expression.
Disciplines
Publication Date
2002
Citation Information
A.M. White, S. Blumerman, B. Naiman and Cynthia Baldwin. "Expression of the bovine high affinity IL-12 receptor b2" Veterinary Immunology and Immunopathology Vol. 84 Iss. 3-4 (2002)
Available at: http://works.bepress.com/cynthia_baldwin/4/