peptide de novo sequencing(2017)
De novo protein sequencing is a process by which the amino acid sequence is deduced without prior knowledge of the DNA or protein sequence. This differs from sequence confirmation, where the protein/DNA sequence is already known and the sequence data obtained is used to confirm that it is correct. Therefore, this tool is especially useful to determine the sequence of novel proteins or antibodies, which are not included in the protein database.
peptide de novo sequencing of an intact protein needs careful experimental design, a combination of different analyses and evaluation of data from mass spectrometry, protein chemistry and bioinformatics. First, the matching or similarity between the de novo sequencing peptide and the database search peptide is a good indication that the database search result is correct. Secondly, the de novo sequencing peptides without significant database hits are possibly novel peptides in the sample, and deserve further examination, such as the finding of unexpected PTM or peptide mutations.
Citation InformationMelissa George. "peptide de novo sequencing" (2017)
Available at: http://works.bepress.com/creativeproteomics/4/